CD45RA antibody

Catalog No. ABIN7880311

The Mouse Monoclonal anti-CD45RA antibody (Clone SPM504) (ABIN7880311) specifically detects CD45RA in IHC (p). The antibody is reactive with Human samples.

Quick Overview for CD45RA antibody (ABIN7880311)

Target: CD45RA

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD45RA antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: SPM504

Product Details anti-CD45RA Antibody

Purpose: CD45RA Antibody

Purification: Protein G affinity chromatography

Immunogen: Stimulated human leukocytes were used as immunogen for this CD45RA antibody.

Isotype: IgG1, kappa

Application Details

Application Notes: Due to variation in protocol and secondary antibody used, the CD45RA antibody may need to be titered for optimal performance.

1. FFPE staining requires boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide, *For IHC use only*

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: The CD45RA antibody (with azide) can be stored at 2-8oC. The azide-free format should be aliquoted and stored at -20oC or colder.

Target Details for CD45RA

Target: CD45RA

Alternative Name: CD45RA

Background: CD45RA is an isoform of the human leukocyte common antigen (CD45). Human CD45 contains three exons which encode peptide segments designated A, B and C, respectively. The differential splicing of the exons generates at least five isoforms, ABC, AB, BC, B and O. This antibody reacts with ABC and BC isoforms. CD45RA is expressed on 40-50 % of peripheral CD4+ T-cells, 50 % of peripheral CD8+ T-cells, B-cells, and leukemic B-cell lines. T-cells expressing CD45RA are naive or virgin T-cells. T-cells expressing CD45RO are memory T-cells. CD45RA and CD45RO define complementary, predominantly non-overlapping populations of resting peripheral T-cells. This MAb is useful in study on the subpopulation of CD4+ or CD8+ T-cells. It can especially be used to differentiate T-cell lymphomas (CD45RO +ve) from B cell lymphomas (CD45RA +ve).

UniProt: P08575