Recombinant PAI1 antibody

Catalog No. ABIN7881956

The Rabbit Monoclonal anti-PAI1 antibody (Clone ABHG-19) (ABIN7881956) specifically detects PAI1 in WB. The antibody is reactive with Human samples.

Quick Overview for Recombinant PAI1 antibody (ABIN7881956)

Target: PAI1 (SERPINE1) (Plasminogen Activator Inhibitor 1 (SERPINE1))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This PAI1 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: ABHG-19

Product Details anti-PAI1 Antibody

Purpose: PAI-1 Antibody / Plasminogen activator inhibitor 1 / SERPINE1

Purification: Affinity purified

Immunogen: A synthetic peptide specific to human PAI1 / SERPINE1 was used as the immunogen for the PAI-1 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the PAI-1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Antibody in PBS with 0.02 % sodium azide, 50 % glycerol and 0.4-0.5 mg/mL BSA

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the PAI-1 antibody at -20oC.

Target Details for PAI1

Target: PAI1 (SERPINE1) (Plasminogen Activator Inhibitor 1 (SERPINE1))

Alternative Name: PAI-1

Background: PAI-1 antibody, also known as Plasminogen activator inhibitor 1 antibody, recognizes a secreted serine protease inhibitor encoded by the SERPINE1 gene and commonly referred to as Serpin E1 and endothelial plasminogen activator inhibitor. Plasminogen activator inhibitor 1 is a member of the serpin superfamily and is localized predominantly to the extracellular space and circulating plasma, where it tightly regulates fibrinolysis. It is highly expressed in endothelial cells, adipocytes, hepatocytes, platelets, smooth muscle cells, and various tumor cell types, with expression levels increasing in response to inflammatory cytokines, hypoxia, metabolic stress, and tissue injury.

Plasminogen activator inhibitor 1 functions as the primary physiological inhibitor of tissue-type plasminogen activator and urokinase-type plasminogen activator, thereby limiting plasmin generation and stabilizing fibrin clots. Through this antifibrinolytic activity, it plays a critical role in thrombosis, wound healing, and extracellular matrix remodeling. Beyond hemostasis, PAI-1 is actively involved in angiogenesis, cell adhesion, migration, and tumor invasion through interactions with vitronectin and the urokinase receptor complex. PAI-1 antibody is frequently used in studies investigating coagulation biology, vascular pathology, and tumor microenvironment signaling.

Structurally, Plasminogen activator inhibitor 1 contains the conserved reactive center loop characteristic of serpin family members, allowing it to form stable inhibitory complexes with target proteases. The protein exists in active, latent, and cleaved conformations, each with distinct functional implications. Binding to vitronectin stabilizes the active conformation and prolongs its half-life in circulation. Within tissues, PAI-1 is detected in the pericellular matrix and extracellular compartment, often co-localizing with integrins and components of the plasminogen activation system during dynamic remodeling processes.

Dysregulation of SERPINE1 expression is strongly associated with human disease. Elevated PAI-1 levels are linked to deep vein thrombosis, myocardial infarction, atherosclerosis, and other cardiovascular disorders characterized by impaired fibrinolysis. Increased expression is also observed in obesity and type 2 diabetes, contributing to metabolic and vascular complications. In oncology, high SERPINE1 expression correlates with poor prognosis in several malignancies, including breast, lung, and colorectal cancers, where it supports tumor cell survival, invasion, and resistance to apoptosis.

PAI-1 antibody supports research into TGF-beta signaling, hypoxia-inducible factor pathways, and inflammatory cytokine networks that regulate SERPINE1 transcription. Developmentally, PAI-1 expression increases during tissue repair and inflammatory responses, reflecting its role in controlled matrix turnover and immune regulation. Clone ABHG-19 recognizes Plasminogen activator inhibitor 1 and is suitable for detecting PAI-1 expression in relevant research applications.

UniProt: P05121

Pathways: p53 Signaling, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Autophagy, Smooth Muscle Cell Migration