GFP antibody (Alexa Fluor 750)

Catalog No. ABIN7937942

The Alpaca Monoclonal anti-GFP antibody is suitable to detect GFP in samples from Aequorea victoria. It has been validated for IF, ICC and FACS.

Quick Overview for GFP antibody (Alexa Fluor 750) (ABIN7937942)

Target: GFP (Green Fluorescent Protein (GFP))

Reactivity: Aequorea victoria

Host: Alpaca

Clonality: Monoclonal

Conjugate: This GFP antibody is conjugated to Alexa Fluor 750

Application: Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Clone: 1H1-1B2

Product Details anti-GFP Antibody (Alexa Fluor 750)

Purpose: Camelid sdAb anti-GFP Alexa Fluor™ 750, Clone(s)1H1 / 1B2

Immunogen: GFP (green fluorescent protein) and common GFP derivatives like EGFP, mEGFP, Sirius, tSapphire, Cerulean, eCFP, mTurquoise, acGFP, Emerald, superecliptic pHluorin, paGFP, superfolder GFP, eYFP, mVenus and Citrine

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

A blend of 2 different sdAbs that bind at different epitopes of the same target protein, two fluorophore molecules are site-specifically coupled to each individual sdAb molecule. The reagent therefore simultaneously targets up to four fluorophores to the protein of interest, which ensures extra-bright signals. Owing to the small size of sdAb, the distance between the target epitope and each fluorophore is below 4 nm. In comparison to conventional detection systems using conventional antibodies, this product can thus improve the localization accuracy by 10-15 nm. Both features - superior brightness and precise fluorophore placement - render this products excellent tools for all microscopy techniques.

Restrictions: For Research Use only

Handling

Concentration: 5 μM

Storage: -80 °C

Storage Comment: -80°C

Target Details for GFP

Target: GFP (Green Fluorescent Protein (GFP))

Alternative Name: GFP