Caspase 8 antibody (AA 388-480)

Catalog No. ABIN7975447

The Rabbit Polyclonal anti-Caspase 8 antibody is suitable to detect Caspase 8 in samples from Mouse. It has been validated for WB.

Quick Overview for Caspase 8 antibody (AA 388-480) (ABIN7975447)

Target: Caspase 8 (CASP8)

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Caspase 8 antibody is un-conjugated

Application: Western Blotting (WB)

Grade: Carrier-free

Product Details anti-Caspase 8 Antibody

Binding Specificity: AA 388-480

Purpose: Anti-Caspase-8/CASP8 Antibody

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: Mouse Caspase 8 shares 83% amino acid (aa) sequence identity with human Caspase 8.

Characteristics: Anti-Caspase-8/CASP8 Antibody. Tested in WB applications. This antibody reacts with Mouse.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived mouse Caspase 8 recombinant protein (Position: S388-P480). Mouse Caspase 8 shares 83% amino acid (aa) sequence identity with human Caspase 8.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Caspase 8

Target: Caspase 8 (CASP8)

Alternative Name: CASP8

Background:

Background: CASP8 is also known as CAP4, MACH or MCH5. This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. This protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Many alternatively spliced transcript variants encoding different isoforms have been described, although not all variants have had their full-length sequences determined.?

Gene Full Name: caspase 8

Sequence Similarities: Belongs to the peptidase C14A family.

Molecular Weight: 55 kDa

Gene ID: 12370

UniProt: O89110

Pathways: Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Activation of Innate immune Response, Tube Formation, Positive Regulation of Endopeptidase Activity, Toll-Like Receptors Cascades