ALIX antibody (AA 2-330) (Biotin)

Catalog No. ABIN8025629

This Mouse Monoclonal antibody specifically detects ALIX in WB, ELISA and IHC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for ALIX antibody (AA 2-330) (Biotin) (ABIN8025629)

Target: ALIX (PDCD6IP) (Programmed Cell Death 6 Interacting Protein (PDCD6IP))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This ALIX antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Clone: 14D10

Product Details anti-ALIX Antibody (Biotin)

Binding Specificity: AA 2-330

Purpose: Anti-ALIX/PDCD6IP Antibody(monoclonal, 14D10) Biotin Conjugated

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Predicted Reactivity: Human PDCD6IP shares?96.7%,95.2% amino?acid? (aa)?sequence?identity?with mouse?and rat PDCD6IP,respectively.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human PDCD6IP recombinant protein (Position: A2-D330). Human PDCD6IP shares?96.7% and 95.2% amino?acid? (aa)?sequence?identity?with mouse?and rat PDCD6IP, respectively.

Isotype: IgG2b

Application Details

Application Notes: Western blot, Optimal dilutions should be determined by end users. Immunohistochemistry (Paraffin-embedded Section), Optimal dilutions should be determined by end users. ELISA, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for ALIX

Target: ALIX (PDCD6IP) (Programmed Cell Death 6 Interacting Protein (PDCD6IP))

Alternative Name: PDCD6IP

Background:

Background: Programmed cell death 6-interacting protein is a protein that in humans is encoded by the PDCD6IP gene. This gene encodes a protein that functions within the ESCRT pathway in the abscission stage of cytokinesis, in intralumenal endosomal vesicle formation, and in enveloped virus budding. Studies using mouse cells have shown that overexpression of this protein can block apoptosis. In addition, the product of this gene binds to the product of the PDCD6 gene, a protein required for apoptosis, in a calcium-dependent manner. This gene product also binds to endophilins, proteins that regulate membrane shape during endocytosis. Overexpression of this gene product and endophilins results in cytoplasmic vacuolization, which may be partly responsible for the protection against cell death. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Gene Full Name: programmed cell death 6 interacting protein

Gene ID: 10015

UniProt: Q8WUM4

Pathways: p53 Signaling, EGFR Signaling Pathway, Sensory Perception of Sound, Cellular Response to Molecule of Bacterial Origin, Tube Formation