Recombinant PAI1 antibody (N-Term)

Catalog No. ABIN8093412

The Mouse Monoclonal anti-PAI1 antibody (Clone rTJA6) (ABIN8093412) specifically detects PAI1 in WB and IHC. The antibody is reactive with Human samples.

Quick Overview for Recombinant PAI1 antibody (N-Term) (ABIN8093412)

Target: PAI1 (SERPINE1) (Plasminogen Activator Inhibitor 1 (SERPINE1))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PAI1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: RTJA6

Product Details anti-PAI1 Antibody

Binding Specificity: N-Term

Purpose: SERPINE1 Antibody / Plasminogen activator inhibitor 1 / PA1-1

Immunogen: Prokaryotic recombinant protein corresponding to a 250 amino acid portion of the N-terminus of the human PAI-1 molecule was used as the immunogen for the SERPINE1/Serpin family E member 1 antibody.

Isotype: IgG2b, kappa

Application Details

Application Notes: 1. Optimal dilution of the SERPINE1/Serpin family E member 1 antibody should be determined by the researcher.

2. This SERPINE1/Serpin family E member 1 antibody is recombinantly produced by expression in CHO cells.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.05 % BSA, 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: SERPINE1/Serpin family E member 1 antibody with sodium azide - store at 2 to 8oC, antibody without sodium azide - store at -20 to -80oC.

Target Details for PAI1

Target: PAI1 (SERPINE1) (Plasminogen Activator Inhibitor 1 (SERPINE1))

Alternative Name: SERPINE1

Background: SERPINE1 antibody, also known as Serpin family E member 1 antibody, recognizes a secreted serine protease inhibitor commonly referred to as Plasminogen activator inhibitor 1 and PAI-1. Serpin family E member 1 is encoded by the SERPINE1 gene located on chromosome 7q22.1 and belongs to the serpin superfamily of protease inhibitors. The protein is primarily localized to the extracellular space and circulating plasma, where it regulates fibrinolysis by inhibiting tissue-type plasminogen activator and urokinase-type plasminogen activator. SERPINE1 is highly expressed in endothelial cells, adipocytes, hepatocytes, platelets, smooth muscle cells, and various tumor cells, with expression induced by inflammatory cytokines, hypoxia, and metabolic stress.

Serpin family E member 1 functions as the principal physiological inhibitor of plasminogen activation, thereby limiting plasmin formation and stabilizing fibrin clots. Through this activity, it plays a central role in thrombosis, wound healing, extracellular matrix remodeling, and vascular homeostasis. SERPINE1 antibody is widely used to investigate coagulation biology, tissue repair mechanisms, and tumor microenvironment dynamics. Beyond its antifibrinolytic function, SERPINE1 interacts with vitronectin and components of the urokinase receptor system to influence cell adhesion, migration, angiogenesis, and tumor invasion.

Structurally, Serpin family E member 1 contains a conserved reactive center loop typical of serpin proteins, enabling the formation of stable inhibitory complexes with target proteases. The protein exists in active, latent, and cleaved conformations, each associated with distinct biological properties. Binding to vitronectin stabilizes the active conformation and prolongs its functional half-life in circulation. Within tissues, SERPINE1 localizes to the pericellular matrix and extracellular compartment, frequently co-localizing with integrins and the plasminogen activation system during dynamic remodeling events.

Dysregulated SERPINE1 expression is associated with numerous pathological conditions. Elevated levels are linked to deep vein thrombosis, myocardial infarction, atherosclerosis, and other cardiovascular disorders characterized by impaired fibrinolysis. Increased expression is also observed in obesity and type 2 diabetes, contributing to metabolic and vascular complications. In cancer, high SERPINE1 expression correlates with poor prognosis in malignancies such as breast, lung, and colorectal cancers, where it supports tumor cell survival, invasion, and resistance to apoptosis.

SERPINE1 antibody supports research into TGF-beta signaling, hypoxia-inducible factor pathways, and inflammatory cytokine networks that regulate SERPINE1 transcription. Developmentally, expression increases during tissue repair and inflammatory responses, highlighting its role in controlled matrix turnover. Clone rTJA6 recognizes Serpin family E member 1 and is suitable for detecting SERPINE1 expression in relevant research applications.

UniProt: P05121

Pathways: p53 Signaling, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Autophagy, Smooth Muscle Cell Migration