HIST1H3A antibody (AA 131-230) (HRP)
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- Target See all HIST1H3A Antibodies
- HIST1H3A (Histone Cluster 1, H3a (HIST1H3A))
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Binding Specificity
- AA 131-230
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Reactivity
- Human, Mouse, Rat, Sheep
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This HIST1H3A antibody is conjugated to HRP
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Application
- ELISA, Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Cross-Reactivity
- Human, Mouse, Rat, Sheep
- Predicted Reactivity
- Dog,Cow,Pig,Horse
- Purification
- Purified by Protein A.
- Immunogen
- KLH conjugated synthetic peptide derived from human HIF3 alpha
- Isotype
- IgG
- Top Product
- Discover our top product HIST1H3A Primary Antibody
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- Application Notes
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WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500 - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 μg/μL
- Buffer
- Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.
- Preservative
- ProClin
- Precaution of Use
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- Handling Advice
- Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
- Storage
- -20 °C
- Storage Comment
- Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- HIST1H3A (Histone Cluster 1, H3a (HIST1H3A))
- Alternative Name
- H3 alpha (HIST1H3A Products)
- Synonyms
- H3/A antibody, H3FA antibody, histone cluster 1 H3 family member a antibody, histone cluster 1, H3a antibody, HIST1H3A antibody, Hist1h3a antibody
- Background
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Synonyms: IPAS, MOP7, PASD7, HIF-3A, bHLHe17, Hypoxia-inducible factor 3-alpha, HIF-3-alpha, HIF3-alpha, Basic-helix-loop-helix-PAS protein MOP7, Class E basic helix-loop-helix protein 17, HIF3-alpha-1, Inhibitory PAS domain protein, Member of PAS protein 7, PAS domain-containing protein 7, HIF3A
Background: Involved in adaptive response to hypoxia. Suppresses hypoxia-inducible expression of HIF1A and EPAS1. Binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters. The complex HIF3A-ARNT activates the transcription of reporter genes driven by HRE. Isoform 4 has a dominant-negative function of inactivating HIF1A-mediated transcription. Isoform 4 attenuates the binding of HIF1A to hypoxia-responsive elements (HRE), thus inhibiting HRE-driven transcription. Hypoxia induces down-regulation of isoform 4, leading to activation of HIF1A in hypoxia. Conversely, upon restoring normoxia, the expression of isoform 4 increases and thereby secure an inhibition of HIF1A activity. Isoform 4 may be a negative regulator of hypoxia-inducible gene expression in the kidney and may be involved in renal tumorigenesis. Functions as an inhibitor of angiogenesis in the cornea (By similarity).
- Gene ID
- 64344
- UniProt
- Q9Y2N7
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