P4HTM antibody (AA 201-300) (HRP)
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- Target See all P4HTM Antibodies
- P4HTM (Prolyl 4-Hydroxylase, Transmembrane (Endoplasmic Reticulum) (P4HTM))
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Binding Specificity
- AA 201-300
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This P4HTM antibody is conjugated to HRP
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Predicted Reactivity
- Human,Mouse,Rat,Pig,Horse
- Purification
- Purified by Protein A.
- Immunogen
- KLH conjugated synthetic peptide derived from human HIFPH4
- Isotype
- IgG
- Top Product
- Discover our top product P4HTM Primary Antibody
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- Application Notes
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WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500 - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 μg/μL
- Buffer
- Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.
- Preservative
- ProClin
- Precaution of Use
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- Handling Advice
- Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
- Storage
- -20 °C
- Storage Comment
- Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- P4HTM (Prolyl 4-Hydroxylase, Transmembrane (Endoplasmic Reticulum) (P4HTM))
- Alternative Name
- HPH4 (P4HTM Products)
- Synonyms
- Ph-4 antibody, RGD1311848 antibody, EGLN4 antibody, HIFPH4 antibody, P4H-TM antibody, PH-4 antibody, PH4 antibody, PHD4 antibody, 4933406E20Rik antibody, AI853847 antibody, BB128974 antibody, prolyl 4-hydroxylase, transmembrane antibody, prolyl 4-hydroxylase, transmembrane (endoplasmic reticulum) antibody, shisa family member 5 antibody, P4htm antibody, P4HTM antibody, SHISA5 antibody
- Background
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Synonyms: EGLN4, H prolyl hydroxylase PH4, H-PH4, H-prolyl hydroxylase 4, HPH4, HPH-4, Hypoxia inducible factor prolyl 4 hydroxylase, Hypoxia inducible factor prolyl hydroxylase, Hypoxia-inducible factor prolyl hydroxylase 4, P4H TM, P4H with transmembrane domain, P4H-TM, P4htm, P4HTM_HUMAN, PH 4, PH4, PHD4, Proline 4 hydroxylase, Prolyl 4 hydroxylase transmembrane endoplasmic reticulum, Prolyl hydroxlase domain containing 4, Transmembrane prolyl 4-hydroxylase.
Background: Catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates HIF1A at 'Pro-402' and 'Pro-564'. May function as a cellular oxygen sensor and, under normoxic conditions, may target HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex.
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