AF4 antibody (AA 31-130) (HRP)

Catalog No. ABIN760385

Product Details anti-AF4 Antibody (HRP)

Target: AF4 (AFF1) (AF4/FMR2 Family, Member 1 (AFF1))

Binding Specificity: AA 31-130

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AF4 antibody is conjugated to HRP

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Predicted Reactivity: Human,Mouse,Rat,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human AF 4 protein

Isotype: IgG

Application Details

Application Notes: IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for AF4

Target: AF4 (AFF1) (AF4/FMR2 Family, Member 1 (AFF1))

Alternative Name: AF4 (AFF1 Products)

Synonyms: AF4 antibody, MLLT2 antibody, PBM1 antibody, 9630032B01Rik antibody, AW319193 antibody, Af4 antibody, Mllt2h antibody, Rob antibody, Mllt2 antibody, AF4/FMR2 family member 1 antibody, AF4/FMR2 family, member 1 antibody, AFF1 antibody, Aff1 antibody

Background:

Synonyms: AF 4, AF 4 protein, AF-4 protein, AF4/FMR2 family member 1, AFF 1, AFF1, AFF1_HUMAN, ALL1 fused gene from chromosome 4, ALL1-fused gene from chromosome 4 protein, FEL, MLLT2, MYELOID/LYMPHOID OR MIXED LINEAGE LEUKEMIA TRANSLOCATED TO 2, MLLT2, Protein AF-4, Protein FEL, Proto-oncogene AF4.

Background: Proto-oncogene AF-4 (or FEL) is a product of a chromosomal aberration of the human gene AFF1, which is associated with acute leukemias. The fusion of AF-4 on chromosome band 4q21 with the mixed lineage leukemia (MLL or HRX) gene on 11q23 results in a MLL-AF-4 chimeric transcription factor in which AF-4 contributes transcriptional effector properties and requires cell-specific accessory factors. MLL is involved in several chromosomal translocations associated with acute myeloid and lymphoid leukemia. The MLL-AF-4 fusion protein is expressed in all normal hematopoietic cells. The expression of MLL-AF-4 influences the production of protein cyclin-dependent kinase inhibitor (CDKN1B), suggesting that inhibition of MLL-AF-4 expression may be a powerful and highly specific treatment of chemotherapy-resistant leukemia.

Gene ID: 4299