FOXO3 antibody (pSer253)

Catalog No. ABIN8121506

This Rabbit Monoclonal antibody specifically detects FOXO3 in WB, IHC, ELISA, IF and IP. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for FOXO3 antibody (pSer253) (ABIN8121506)

Target: FOXO3 (Forkhead Box O3 (FOXO3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This FOXO3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP)

Clone: AB1658

Product Details anti-FOXO3 Antibody

Binding Specificity: pSer253

Purpose: FOXO3A (phospho Ser253) Rabbit mAb

Specificity: The antibody detects endogenous levels of FOXO3A protein only when phosphorylated at Ser253. The antibody does not cross-react with FOXO3A phosphorylated at other sites.

Purification: The antibody was purified by protein A affinity chromatography.

Immunogen: Recombinant protein (or fragment).

Isotype: IgG

Application Details

Application Notes: WB, 1:1000-1:5000 | IHC, 1:200-1:1000 | IF, 1:200-1:1000 | IP, 0. 5 μg-4 μg antibody for 200 μg-400 μg extracts of whole cells. | ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The antibody is provided in liquid form in phosphate - buffered saline with 50 % glycerol, 0.05 % BSA, and 0.05 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze / thaw cycles

Storage: -20 °C

Storage Comment: Store at-20°C. Avoid freeze / thaw cycles.

Target Details for FOXO3

Target: FOXO3 (Forkhead Box O3 (FOXO3))

Alternative Name: FOXO3A

Background:

Synonyms: FOXO3, FKHRL1, FOXO3A, Forkhead box protein O3, AF6q21 protein, Forkhead in rhabdomyosarcoma-like 1

Background: This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. This gene likely functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Jul 2008],

Gene Name: FOXO3

Protein Name: FOXO3A (phospho Ser253)

Gene ID: 2309

UniProt: O43524

Pathways: Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Carbohydrate Homeostasis