PIK3CA antibody (AA 1000-1068)

Catalog No. ABIN8123754

The Mouse Monoclonal anti-PIK3CA antibody is suitable to detect PIK3CA in samples from Human. It has been validated for IHC.

Quick Overview for PIK3CA antibody (AA 1000-1068) (ABIN8123754)

Target: PIK3CA (Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide (PIK3CA))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PIK3CA antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: AB1436

Product Details anti-PIK3CA Antibody

Binding Specificity: AA 1000-1068

Purpose: PIK3CA (H1047R) mouse mAb

Specificity: The antibody can specifically recognize the h1047r mutation of human PIK3CA protein, and does not react with wild-type PIK3CA protein.

Purification: The antibody was purified by affinity chromatography using a specific immunogen.

Immunogen: A synthetic peptide corresponding to the amino acid region 1000 - 1068 of the human PIK3CA H1047R protein.

Isotype: IgG

Application Details

Application Notes: IHC, 1:200-1:400

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The antibody is provided in liquid form in phosphate - buffered saline with 50 % glycerol, 0.05 % BSA, and 0.05 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze / thaw cycles

Storage: -20 °C

Storage Comment: Store at-20°C. Avoid freeze / thaw cycles.

Target Details for PIK3CA

Target: PIK3CA (Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide (PIK3CA))

Alternative Name: PIK3CA

Background:

Synonyms: 5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha, 5-bisphosphate 3-kinase catalytic subunit alpha isoform, caPI3K, CLOVE, CWS5, MCAP, MCM, MCMTC, MGC142161, MGC142163, p110 alpha, p110alpha, Phosphatidylinositol 3 kinase catalytic alpha polypeptide, Phosphatidylinositol 3 kinase catalytic 110 KD alpha, Phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit alpha, Phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit alpha isoform, Phosphatidylinositol 4, 5 bisphosphate 3 kinase 110 kDa catalytic subunit alpha, Phosphatidylinositol-4, Phosphoinositide 3 kinase catalytic alpha polypeptide, PI 3 Kinase catalytic subunit alpha, PI3 kinase p110 subunit alpha, PI3-kinase subunit alpha, PI3K, PI3K-alpha, PI3KC A, PIK3C A, Pik3ca, PK3CA, PK3CA_HUMAN, PtdIns 3 kinase p110, PtdIns-3-kinase subunit alpha, PtdIns-3-kinase subunit p110-alpha, Serine/threonine protein kinase PIK3CA

Background: The PIK3CA gene is located at 3Q26. 3 and encodes a 124 kDa protein. The protein is composed of 85 kDa regulatory subunit and 110 kDa catalytic subunit. The most common mutations of PIK3CA occur in exon 9 and exon 20. H1047R, one of the most common mutations occurs in exon 20. PIK3CA plays an important role in the PI3K/AKT/mTOR signaling pathway, which can be activated by this mutation, closely related to the occurrence of tumors and disease progression. In non-small cell lung cancer, H1047R mutantion can phosphorylate Akt and activate downstream pathways to promote the survival and expansion of tumor cells.

Gene Name: PIK3CA

Protein Name: PIK3CA H1047R

Gene ID: 5290

UniProt: P42336

Pathways: PI3K-Akt Signaling, RTK Signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Inositol Metabolic Process, Hepatitis C, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, VEGF Signaling