HRAS antibody (AA 51-150) (HRP)

Catalog No. ABIN763385

The Rabbit Polyclonal anti-HRAS antibody has been validated for WB, ELISA and IHC (fro). It is suitable to detect HRAS in samples from Human.

Quick Overview for HRAS antibody (AA 51-150) (HRP) (ABIN763385)

Target: HRAS (HRas proto-oncogene, GTPase (HRAS))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HRAS antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-HRAS Antibody (HRP)

Binding Specificity: AA 51-150

Cross-Reactivity: Human

Predicted Reactivity: Mouse,Rat,Cow,Horse,Chicken

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human HRAS+KRAS

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for HRAS

Target: HRAS (HRas proto-oncogene, GTPase (HRAS))

Alternative Name: HRAS+KRAS

Background:

Synonyms: p21ras, Transforming protein p21, GTPase HRas, GTPase KRas, HRas, HRAS1, KRas, KRAS2, RASH, RASK, RASH_HUMAN, RASK_HUMAN.

Background: The KRAS gene encodes the human cellular homolog of a transforming gene isolated from the Kirsten rat sarcoma virus. The RAS proteins are GDP/GTP-binding proteins that act as intracellular signal transducers. The most well-studied members of the RAS (derived from 'RAt Sarcoma' virus) gene family include KRAS, HRAS, and NRAS. These genes encode immunologically related proteins with a molecular mass of 21 kD and are homologs of rodent sarcoma virus genes that have transforming abilities. While these wildtype cellular proteins in humans play a vital role in normal tissue signaling, including proliferation, differentiation, and senescence, mutated genes are potent oncogenes that play a role in many human cancers.

Gene ID: 3265

Pathways: p53 Signaling, MAPK Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Hepatitis C, Autophagy, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, Regulation of long-term Neuronal Synaptic Plasticity, VEGF Signaling, BCR Signaling