ENOS antibody (AA 1181-1194)
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- Target See all ENOS (NOS3) Antibodies
- ENOS (NOS3) (Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3))
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Binding Specificity
- AA 1181-1194
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Reactivity
- Human, Rabbit, Monkey
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This ENOS antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Brand
- IHC-plus™
- Specificity
- Recognizes eNOS/NOS III, Mr ~135kD. Species cross-reactivity: Human and bovine. Predicted to cross-react with mouse, guinea pig, porcine, rat and canine based on sequence homology.
- Predicted Reactivity
- Percent identity by BLAST analysis: Human, Gorilla, Monkey, Elephant, Rabbit (100%) Mouse, Rat, Hamster, Panda, Bovine, Guinea pig (93%) Dog, Bat, Pig (86%).
- Purification
- Protein A purified
- Immunogen
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KLH-conjugated, synthetic peptide corresponding to aa1181-1194 (C-QERQLRGAVPWAFD) of human eNOS/NOS III. The immunizing sequence has 10/11 identical amino acids in mouse, guinea pig, bovine, porcine, rat and canine. Percent identity by BLAST analysis: Human, Gorilla, Monkey, Elephant, Rabbit (100%), Mouse, Rat, Hamster, Panda, Bovine, Guinea pig (93%), Dog, Bat, Pig (86%).
Type of Immunogen: Synthetic peptide - KLH conjugated - Isotype
- IgG
- Top Product
- Discover our top product NOS3 Primary Antibody
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- Application Notes
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Approved: IHC, IHC-P (5 μg/mL), WB
Usage: Suitable for use in Western Blot and IHC-P. Western Blot Analysis: 0.5-2 μg/mL of this lot detected eNOS/NOS III in RIPA lysates from HUVEC cells. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, 30 % glycerol
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing
- Storage
- 4 °C,-20 °C
- Storage Comment
- May be stored at 4°C for short-term only. For long-term storage and to avoid freeze-thaw cycles, aliquot and store at -20°C. Aliquots are stable for at least 1 year at -20°C.
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- Target
- ENOS (NOS3) (Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3))
- Alternative Name
- NOS3 / eNOS (NOS3 Products)
- Synonyms
- ECNOS antibody, eNOS antibody, 2310065A03Rik antibody, Nos-3 antibody, ecNOS antibody, EC-NOS antibody, NOSIII antibody, cNOS antibody, NOS antibody, NOS3 antibody, eNos antibody, ENOS antibody, nitric oxide synthase 3 antibody, nitric oxide synthase 3, endothelial cell antibody, NOS3 antibody, Nos3 antibody, nos3 antibody
- Background
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Name/Gene ID: NOS3
Synonyms: NOS3, Constitutive NOS, CNOS, Endothelial NOS, ECNOS, NOS type III, NOSIII, EC-NOS, ENOS, Nitric oxide synthase 3 - Gene ID
- 4846
- UniProt
- P29474
- Pathways
- ACE Inhibitor Pathway, Regulation of Systemic Arterial Blood Pressure by Hormones, Cellular Response to Molecule of Bacterial Origin, Myometrial Relaxation and Contraction, Signaling Events mediated by VEGFR1 and VEGFR2, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling
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