Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Sequence
CQERQLRGAV PWAFD Remarks: The immunizing sequence has 10/11 identical amino acids in Mouse, Guinea Pig, Bovine, Porcine, Rat and Canine. Antigen Modificati on: aa1181-1194
Specificity
Recognizes eNOS/NOS III, Mr ~135 kDa.
Cross-Reactivity (Details)
Species reactivity (expected):Bovine, Dog, Guinea pig, Mouse, Porcine, Rat. Species reactivity (tested):Human.
Purification
Protein A Chromatography.
Immunogen
kLH-conjugated, synthetic peptide corresponding to amino acids 1181-1194 of Human eNOS/NOS III.
Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Multiple transcript variants encoding different isoforms have been found for this gene.Synonyms: Constitutive NOS, EC-NOS, Endothelial NOS, NOS type III, Nitric oxide synthase, eNOS, endothelial