This Transferrin Receptor antibody is un-conjugated
Application
Flow Cytometry (FACS)
Specificity
This antibody recognizes the CD71 cell surface antigen, a 190 kDa homodimeric glycoprotein expressed by proliferating cells. CD71 acts as Receptor for Transferrin. Cross reacts with Rhesus Monkey, Mustelid.
Purification
Affinity Chromatography on Protein G
Endotoxin Level
Low Endotoxin
Immunogen
KG1 cell line. Remarks: Spleen cells from immunised BALB/c mice were fused with cells of the mouse NSI myeloma cell line.
Optimal working dilution should be determined by the investigator.
Restrictions
For Research Use only
Concentration
1.0 mg/mL
Buffer
PBS without preservatives
Preservative
Without preservative
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
The transferrin receptor has been structurally characterized as a sulfide bound dimer of identical glycoprotein subunits of 95 kDa. The transferrin receptor is not present on resting blood lymphocytes. On PBL, the receptor appears after activation. The expression of transferrin receptor is coordinately regulated with cell growth. Present on T and B cell lines. The soluble (or serum) transferrin receptor (sTfR) is a circulating truncated form of the membrane receptor protein, it is an 85 kDa glycoprotein forming in serum a 320 kDa complex with diferric transferrin.The most important clinical use of the sTfR determination is in the differential diagnosis between iron deficiency anaemia and the anaemia of chronic disease.Synonyms: TfR1, Transferrin receptor protein 1, p90