PDGFRA antibody (Platelet-Derived Growth Factor Receptor, alpha Polypeptide) (AA 1035-1053)

Details for Product anti-PDGFRA Antibody No. ABIN790499, Supplier: Log in to see
Antigen
  • AI115593
  • CD140a
  • Pdgfr-2
  • APDGFR
  • PDGFACE
  • CD140A
  • PDGFR-2
  • PDGFR2
  • RHEPDGFRA
  • PDGFR
  • PDGFR-A
  • platelet derived growth factor receptor alpha
  • platelet derived growth factor receptor, alpha polypeptide
  • platelet-derived growth factor receptor, alpha polypeptide L homeolog
  • platelet-derived growth factor receptor, alpha polypeptide
  • PDGFRA
  • Pdgfra
  • pdgfra.L
Alternatives
anti-Human PDGFRA antibody for Enzyme Immunoassay
Epitope
AA 1035-1053
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7
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2
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1
1
1
1
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1
1
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Reactivity
Bat, Chicken, Cow (Bovine), Dog (Canine), Hamster, Horse (Equine), Human, Monkey, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Xenopus laevis
489
274
179
5
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2
2
2
1
1
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1
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Host
Rabbit
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143
49
3
1
Clonality
Polyclonal
Conjugate
This PDGFRA antibody is un-conjugated
35
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6
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2
2
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1
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Application
Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
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49
14
14
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14
9
3
2
2
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1
Options
Supplier
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Supplier Product No.
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Brand IHC-plus™
Immunogen Synthetic peptide corresponding to amino acids 1035-1053 (C-GKRNRHSSQTSEESAIETG) of human PDGF (KLH-conjugated). Receptor alpha. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Bat, Dog, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Lizard, Xenopus, Pufferfish (100%), Stickleback (95%), Zebrafish (89%).

Type of Immunogen: Synthetic peptide - KLH conjugated
Isotype IgG
Specificity Recognizes human PDGF Receptor Type A, doublet, Mr 150/170kD. Species Crossreactivity: mouse. Species Sequence Homology: frog, chicken and zebra fish.
Predicted Reactivity Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Bat, Dog, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Lizard, Xenopus, Pufferfish (100%) Stickleback (95%) Zebrafish (89%).
Purification Immunoaffinity purified
Plasmids, Primers & others Plasmids, Primers & others PDGFRA products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name PDGFRA / PDGFR Alpha (PDGFRA Antibody Abstract)
Background Name/Gene ID: PDGFRA
Subfamily: PDGF Receptor
Family: Protein Kinase

Synonyms: PDGFRA, CD140A, CD140a antigen, PDGFalphaR, PDGFR2, PDGFRalpha, AlphaPDGFR, RHEPDGFRA, PDGF receptor alpha, PDGFR-2, PDGFRA/BCR fusion, APDGFR, Pdgf alpha-receptor, PDGF-R-alpha, PDGFR-alpha
Gene ID 5156
UniProt P16234
Pathways RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Platelet-derived growth Factor Receptor Signaling
Application Notes Approved: IHC, IHC-P (1:100), IP, WB (1:250 - 1:1000)

Usage: Suitable for use in Western Blot and Immunoprecipitation. Western Blot: 1:250-1:1000 dilution detected PDGF Receptor a doublet (150/170kD) in 3T3/NIH cell lysate. 3T3/NIH cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-PDGF Receptor a (1:500 dilution). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: 5-10 μL immunoprecipitates PDGF Receptor a from 500 μg of 3T3/NIH RIPA lysate.
Comment

Target Species of Antibody: Human

Restrictions For Research Use only
Format Liquid
Concentration Lot specific
Buffer 0.1 M Tris-glycine,  pH 7.4, 150 mM sodium chloride, 0.05 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeat freeze-thaw cycles.
Storage 4 °C
Storage Comment May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Supplier Images
 image for anti-Platelet-Derived Growth Factor Receptor, alpha Polypeptide (PDGFRA) (AA 1035-1053) antibody (ABIN790499) Human Brain, Cerebellum: Formalin-Fixed, Paraffin-Embedded (FFPE)
Immunohistochemistry (IHC) image for anti-Platelet-Derived Growth Factor Receptor, alpha Polypeptide (PDGFRA) (AA 1035-1053) antibody (ABIN790499) Anti-PDGFRA antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin...
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