Ki-67 antibody

Catalog No. ABIN799922

Product Details anti-Ki-67 Antibody

Target: Ki-67 (MKI67) (Antigen Identified By Monoclonal Antibody Ki-67 (MKI67))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Ki-67 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: Recognizes human Ki-67. Reacts with a nuclear antigen present in proliferating human cells. Can be used to evaluate the Ki-67 labeling index in various tumors.

No Cross-Reactivity: Rat (Rattus)

Purification: Protein A purified

Immunogen: Synthetic peptide corresponding to an internal region of human Ki-67 (within residues 1550-1700). Cellular Localization: Nuclear

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: ICC (1:50), IF (1:20), IHC, IHC-Fr, IHC-P

Usage: Suitable for use in Immunohistochemistry, Immunocytochemistry and Immunofluorescence. Immunohistochemistry (Frozen/Paraffin): Formalin-fixed paraffin-embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer,  pH 6.0 prior to immunostaining. This antibody will not work without optimal antigen retrieval. We suggest an incubation period of 30 minutes at RT and to use DAB to stain the protein (immunofluorescence may give problems as the protein is nuclear). Immunocytochemistry: 1:50. Immunofluorescence: 1:20. Positive control: Tonsil.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.05 % sodium azide, 30 % glycerol

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Ki-67

Target: Ki-67 (MKI67) (Antigen Identified By Monoclonal Antibody Ki-67 (MKI67))

Alternative Name: MKI67 / Ki67 (MKI67 Products)

Synonyms: MKI67 antibody, kia antibody, mki67 antibody, MGC132156 antibody, D630048A14Rik antibody, Ki-67 antibody, Ki67 antibody, KIA antibody, MIB-1 antibody, marker of proliferation Ki-67 antibody, marker of proliferation Ki-67 L homeolog antibody, marker of proliferation Ki-67 S homeolog antibody, antigen identified by monoclonal antibody Ki 67 antibody, antigen identified by monoclonal antibody Ki-67 antibody, nucleolar protein interacting with the FHA domain of MKI67 antibody, MKI67 antibody, mki67 antibody, mki67.L antibody, mki67.S antibody, Mki67 antibody, NIFK antibody

Background: Name/Gene ID: MKI67

Synonyms: MKI67, Antigen KI-67, Ki67, KIA

Gene ID: 4288

UniProt: P46013

Pathways: Glycosaminoglycan Metabolic Process