p63alpha antibody

Catalog No. ABIN865458

Product Details

Target: p63alpha

Reactivity: Human, Monkey, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Purification: Ascitic fluid

Immunogen: Synthesized peptide of human p63alpha.

Clone: 4E5

Isotype: IgG1

Application Details

Application Notes: WB: 1/500 - 1/2000, IHC: 1/200-1/1000 , ELISA: Propose dilution 1/10000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100g/100l

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Target Details

Target: p63alpha

Background: The p63 gene is a homologue of the p53 tumor suppressor gene. Like p53, p63 contains a transactivation (TA) domain induce the transcription of target genes, a DNA binding domain, and an oligomerization domain (OD), used to form tetramers. In contrast to p53, the p63 gene encodes for at least six major isotypes. Three isotypes (TAp63alpha, TAp63beta, and TAp63gamma) contain the transactivating (TA) domain and are able to transactivate p53 report genes and induce apoptosis. In contrast, the other three isotypes (deltaNp63alpha, deltaNp63beta, deltaNp63gamma) are transcribed from an internal promoter localized within intron3, lack the TA domain, and act as dominant-negatives to suppress transactivation by both p53 and TAp63 isotypes. p63 is highly expressed in the basal cells of the epithelium significant for proper limb outgrowth and morphogenesis.4 In differentiating tissues, p63 is crucial for maintaining the stem cell identity of the basal cells, and is indispensable for correct development of the skin as well as the limb. p63-deficient mice lack all squamous epithelia and their derivatives, including hair, whiskers, teeth, as well as mammary, lacrimal, and salivary glands. Tissue specificity: Widely expressed, notably in heart, kidney, placenta, prostate, skeletal muscle, testis and thymus, although the precise isoform varies according to tissue type. Synonyms: P51B, KET, p63

Molecular Weight: 51kDa

Gene ID: 8626