The antibody detects endogenous levels of total NCAM protein.
Purification
Affinity purified
Immunogen
Peptide sequence around AA 850-854 (Q-T-K-E-N) derived from Human CD56 (NCAM) . Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.
NCAM (neural cell adhesion molecule, CD56) is an adhesion glycoprotein with five extracellular immunoglobulin-like domains followed by two fibronectin type III repeats. Structural diversity is introduced by alternative splicing resulting in different cytoplasmic domains (1). NCAM mediates neuronal attachment, neurite extension and cell-cell interactions through homo and heterophilic interactions. PSA (polysialic acid) post-translationally modifies NCAM and increases the metastatic potential of small cell lung carcinoma, Wilms+ tumor, neuroblastoma and rhabdomyosarcoma (2). CD56 and CD16 are commonly used to identify NK cells although some cells with the T cell markers CD3 and CD4 also express CD56