PSMD14 antibody (AA 101-200) (Alexa Fluor 488)

Catalog No. ABIN905319

Product Details anti-PSMD14 Antibody (Alexa Fluor 488)

Target: PSMD14 (Proteasome (Prosome, Macropain) 26S Subunit, Non-ATPase, 14 (PSMD14))

Binding Specificity: AA 101-200

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PSMD14 antibody is conjugated to Alexa Fluor 488

Application: Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Predicted Reactivity: Human,Mouse,Rat,Cow,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human MAD1

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for PSMD14

Target: PSMD14 (Proteasome (Prosome, Macropain) 26S Subunit, Non-ATPase, 14 (PSMD14))

Alternative Name: Mad1 (PSMD14 Products)

Synonyms: PAD1 antibody, POH1 antibody, RPN11 antibody, 2610312C03Rik antibody, 3200001M20Rik antibody, AA986732 antibody, Pad1 antibody, Poh1 antibody, rpm11 antibody, zgc:162272 antibody, proteasome 26S subunit, non-ATPase 14 antibody, proteasome (prosome, macropain) 26S subunit, non-ATPase, 14 antibody, 26S proteasome non-ATPase regulatory subunit 14 antibody, proteasome 26S subunit, non-ATPase 14 L homeolog antibody, 26S proteasome-associated pad1 homolog antibody, PSMD14 antibody, Psmd14 antibody, rpn-11 antibody, psmd14 antibody, psmd14.L antibody

Background:

Synonyms: mitosis arrest deficiency 1, hMAD1, HsMAD1, MAD1L1, Mitotic arrest deficient 1, Mitotic checkpoint MAD1 protein homolog, Mitotic spindle assembly checkpoint protein MAD1, PIG9, Tax binding protein 181, TP53I9 , Tumor protein p53 inducible protein 9, TXBP181, MD1L1_HUMAN.

Background: spindle microtubules. The checkpoint works by inhibiting the activity of the anaphase promoting complex, thereby preventing the degradation of several cell cycle regulators. Like other spindle checkpoint mutants, MAD1 loss-of-function mutants are sensitive to benomyl and cannot delay cell division in response to spindle depolymerization. Mad1p becomes hyperphosphorylated upon spindle depolymerization.

Gene ID: 8379

Pathways: Mitotic G1-G1/S Phases, DNA Replication, M Phase, Positive Regulation of Endopeptidase Activity, Synthesis of DNA, Ubiquitin Proteasome Pathway