c-MYC antibody (HRP)
Quick Overview for c-MYC antibody (HRP) (ABIN920765)
Target
See all c-MYC (MYC) AntibodiesReactivity
Host
Clonality
Conjugate
Application
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Application Notes
- ELISA: Use at 1:10,000. Western blot: Use at 1:2,000
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Restrictions
- For Research Use only
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Format
- Liquid
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Buffer
- 50 mM HEPES pH 7.0, 0.1 M Sodium Chloride with 0.2% BSA and 0.1% thimerosal.
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Preservative
- Thimerosal (Merthiolate)
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Precaution of Use
- This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- 4 °C
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- c-MYC (MYC) (Myc Proto-Oncogene protein (MYC))
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Alternative Name
- C-Myc
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Background
- Chickens were immunized with the C-Myc peptide conjugated with KLH. After multiple immunizations in Freund's adjuvant, eggs were collected. Antibodies were immunoaffinity- purified using the peptide immobilized on a solid phase. The antibodies were conjugated with horseradish peroxidase (HRP). Specification: ELISA: Antibody specificity was verified by ELISA against the peptide conjugated to BSA. A 1:20,000 dilution of the antibody gave an OD = 1.0 in a 15 minute reaction using TMB as the substrate. Appropriate specificity controls were run.
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Pathways
- p53 Signaling, Cell Division Cycle, Sensory Perception of Sound, Transition Metal Ion Homeostasis, Mitotic G1-G1/S Phases, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus, Warburg Effect
Target
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