P-Selectin antibody

Catalog No. ABIN933999

Product Details anti-P-Selectin Antibody

Target: P-Selectin (SELP) (Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP))

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This P-Selectin antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Immunogen: CD62 P antibody was raised in mouse using mouse myeloma cell line NS0-derived recombinant mouse PSelectin/CD62 as the immunogen.

Clone: 127933-M101281

Isotype: IgG2b

Application Details

Application Notes: ELISA: 2-8 µg/mL, WB: 1 µg/mL
Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with sterile PBS.

Concentration: Lot specific

Buffer: Supplied lyophilized from PBS, with 5 % Trehalose.

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C until reconstitution. Following reconstitution product may be stored at 4 °C in the short term. For long term storage aliquot and freeze at -20 °C.

Target Details for P-Selectin

Target: P-Selectin (SELP) (Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP))

Alternative Name: CD62P (SELP Products)

Synonyms: PSELECT antibody, CD62 antibody, CD62P antibody, GMP140 antibody, GRMP antibody, LECAM3 antibody, PADGEM antibody, PSEL antibody, GMP-140 antibody, Grmp antibody, selectin P antibody, selectin, platelet antibody, Selp antibody, SELP antibody

Background: Mouse P-Selectin (GMP140, LECAM3, PADGEM, CD62P), a member of the Selectin family, is a cell surface glycoprotein expressed by activated platelets and endothelial cells. P-Selectin is translocated to the cell surface within minutes, from alpha granules of platelets or WeibelPalade bodies of endothelial cells, following stimulation with thrombin, histamine, PMA or peroxides. P-Selectin binds to a 106 kDa protein present on myeloid cells, neutrophils, monocytes and lymphocytes, termed PSGL 1 (PSelectin glycoprotein ligand). P-Selectin plays a role in the adhesion of leukocytes and neutrophils to the endothelium. Acting in cooperation with L-Selectin, P-Selectin mediates the initial interaction of circulating leukocytes with endothelial cells that produces a characteristic ‘rolling’ of the leukocytes on the endothelium. Synonyms: Monoclonal CD62P antibody, Anti-CD62P antibody, P-Selectin antibody.