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Blood Group Lewis B antibody

Reactivity: Human IHC (p), WB, ELISA Host: Mouse Monoclonal 2-25LE unconjugated
Catalog No. ABIN93925
  • Target See all Blood Group Lewis B products
    Blood Group Lewis B
    Reactivity
    • 13
    • 11
    • 9
    Human
    Host
    • 13
    Mouse
    Clonality
    • 13
    Monoclonal
    Conjugate
    • 6
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Blood Group Lewis B antibody is un-conjugated
    Application
    • 12
    • 9
    • 9
    • 4
    • 2
    • 1
    • 1
    Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB), ELISA
    Specificity
    The antibody 2-25LE reacts with Lewisb blood group antigen, a carbohydrate determinant carried on both glycolipids and glycoproteins, detected on the surface of erythrocytes, certain epithelial cells, and in secretions of certain individuals.
    Cross-Reactivity (Details)
    Other not tested, Human
    Purification
    Purified by protein-A affinity chromatography.
    Purity
    > 95 % (by SDS-PAGE)
    Immunogen
    Mucin isolated by size exclusion chromatography from an ovarian cyst fluid (pure endocervical type according to the Fenoglio´s classification).
    Clone
    2-25LE
    Isotype
    IgG1
  • Application Notes
    Immunohistochemistry (paraffin sections): Recommended dilution: 5-10 μg/mL, positive control: colorectal adenocarcinoma, heat mediated antigen retrieval (sodium citrate), mAb incubation: 1 hour / RT, detection DAB.
    Restrictions
    For Research Use only
  • Concentration
    1 mg/mL
    Buffer
    Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Do not freeze.
    Storage
    4 °C
    Storage Comment
    Store at 2-8°C. Do not freeze.
  • Creuzot-Garcher, Guerzider, Assem, Bron, Delannoy, Bara: "Alteration of sialyl Lewis epitope expression in pterygium." in: Investigative ophthalmology & visual science, Vol. 40, Issue 8, pp. 1631-6, (1999) (PubMed).

    "First International Workshop on Monoclonal Antibodies Against Red Blood Cell and Related Antigens. Paris, September 21-24, 1987. Vol. II: Other blood group systems." in: Revue française de transfusion et immuno-hématologie, Vol. 31, Issue 2, pp. 115-486, (1989) (PubMed).

  • Target
    Blood Group Lewis B
    Abstract
    Blood Group Lewis B Products
    Synonyms
    MUC5 antibody, TBM antibody, leB antibody, mucin 5AC, oligomeric mucus/gel-forming antibody, MUC5AC antibody
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