HLA-DR antibody

Catalog No. ABIN94340

The Mouse Monoclonal anti-HLA-DR antibody has been validated for FACS and IP. It is suitable to detect HLA-DR in samples from Human. There are 2+ publications available.

Quick Overview for HLA-DR antibody (ABIN94340)

Target: HLA-DR

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This HLA-DR antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP)

Clone: HL-39

Product Details anti-HLA-DR Antibody

Purpose: Anti-HLA-DR Purified

Specificity: The antibody HL-39 recognizes an extracellular common epitope on human HLA-DR, which is dependent on the association of alpha and beta chains. Recognized epitope is conserved after fixation with glutaraldehyde and formaldehyde. DR is the isotype of human MHC Class II molecules expressed on antigen-presenting cells (APC, dendritic cells, B lymphocytes, monocytes, macrophages).

Cross-Reactivity (Details): Human

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: Raji Burkitt's lymphoma cell line

Isotype: IgG3

Application Details

Application Notes: Flow cytometry: Recommended dilution: 0.5-4 μg/mL.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-HLA-DR antibody (ABIN94340)

Hovden, Karlsen, Jonsson, Aarstad, Appel: "Maturation of monocyte derived dendritic cells with OK432 boosts IL-12p70 secretion and conveys strong T-cell responses." in: BMC immunology, Vol. 12, pp. 2, (2011) (PubMed).

Horejsí, N?mec, Angelisová, Kristofová, Gorga, Hilgert: "Characterization of seven new monoclonal antibodies against human DR, DR + DP and DQ1 + DQ3 antigens." in: Tissue antigens, Vol. 28, Issue 5, pp. 288-97, (1987) (PubMed).

Target Details for HLA-DR

Target: HLA-DR

Alternative Name: HLA-DR

Background: HLA-DR, a member of MHC class II glycoproteins, that bind intracellularly processed peptides and present them to the Th cells, is composed of 36 kDa alpha chain and 27 kDa beta chain, both anchored in the plasma membrane. Together with other MHC II molecules HLA-DR plays a central role in the immune system.

Pathways: Human Leukocyte Antigen (HLA) in Adaptive Immune Response