HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) antibody

Details for Product No. ABIN94359
Antigen
Reactivity
Human
323
9
8
5
1
1
1
1
Host
Mouse
219
82
26
5
3
1
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
43
41
25
18
11
5
2
2
2
2
2
2
1
1
1
1
1
Application
ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunoprecipitation (IP)
184
171
170
110
62
47
36
32
22
18
14
13
8
3
2
2
2
1
1
Options
Immunogen HLA-B27 transgenic mice were imunized with H-2 identical murine cells transfected with and expressing genes encoding HLA-G and human beta2-microglobulin.
Clone 01G
Isotype IgG1
Specificity The antibody 01G recognizes membrane-bound form of HLA-G (full-length HLA-G1), but not soluble forms. HLA-G belongs to the MHC Class I molecules (MHC Class Ib, nonclassical) and it is expressed on the surface of trophoblast cells.
No Cross-Reactivity Mouse (Murine), Rat (Rattus)
Cross-Reactivity (Details) Human
Purification Purified by protein-A affinity chromatography
Purity > 95 % (by SDS-PAGE)
Antigen
Alternative Name HLA-G (HLAG Antibody Abstract)
Pathways Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Human Leukocyte Antigen (HLA) in Adaptive Immune Response
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Product cited in: Wiendl, Feger, Mittelbronn, Jack, Schreiner, Stadelmann, Antel, Brueck, Meyermann, Bar-Or, Kieseier, Weller: "Expression of the immune-tolerogenic major histocompatibility molecule HLA-G in multiple sclerosis: implications for CNS immunity." in: Brain : a journal of neurology, Vol. 128, Issue Pt 11, pp. 2689-704, 2005 (PubMed).

Poláková, Bandzuchová, Hofmeister, Weiss, Hutter, Russ: "Binding analysis of HLA-G specific antibodies to hematopoietic cells isolated from leukemia patients." in: Neoplasma, Vol. 50, Issue 5, pp. 331-8, 2003 (PubMed).

Poláková, Krcová, Kuba, Russ: "Analysis of HLA-G expression in malignant hematopoetic cells from leukemia patients." in: Leukemia research, Vol. 27, Issue 7, pp. 643-8, 2003 (PubMed).

Poláková, Russ: "Expression of the non-classical HLA-G antigen in tumor cell lines is extremely restricted." in: Neoplasma, Vol. 47, Issue 6, pp. 342-8, 2001 (PubMed).

Real, Cabrera, Canton, Oliva, Ruiz-Cabello, Garrido: "Looking for HLA-G expression in human tumours." in: Journal of reproductive immunology, Vol. 43, Issue 2, pp. 263-73, 1999 (PubMed).

Real, Cabrera, Collado, Jimenez, Garcia, Ruiz-Cabello, Garrido: "Expression of HLA G in human tumors is not a frequent event." in: International journal of cancer. Journal international du cancer, Vol. 81, Issue 4, pp. 512-8, 1999 (PubMed).