HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) antibody (FITC)

Details for Product No. ABIN94377
Antigen
Reactivity
Human
322
9
8
5
1
1
1
1
Host
Mouse
219
81
26
5
3
1
Clonality (Clone)
Monoclonal ()
Conjugate
FITC
42
41
25
18
11
5
2
2
2
2
2
2
1
1
1
1
1
Application
ELISA, Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunoprecipitation (IP)
184
170
170
110
62
47
36
33
22
18
14
13
8
3
2
2
2
1
1
Options
Immunogen Recombinant human HLA-G refolded with beta2-microglobulin and peptide.
Clone MEM-G-11
Isotype IgG1
Specificity The antibody MEM-G/11 recognizes HLA-G1 antigen. HLA-G belongs to the MHC Class I molecules (MHC Class Ib, nonclassical) and it is expressed on the surface of trophoblast cells.
Cross-Reactivity (Details) Human
Antigen
Alternative Name HLA-G (HLAG Antibody Abstract)
Pathways Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Human Leukocyte Antigen (HLA) in Adaptive Immune Response
Application Notes The reagent is designed for Flow Cytometry analysis of cells expressing HLA-G molecule on the cell surface. Suggested working dilution is 1:500. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator.
Comment

The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC.

Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Avoid prolonged exposure to light.
Storage 4 °C
Storage Comment Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Supplier Images
Image no. 1 for anti-HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) antibody (FITC) (ABIN94377) Surface staining of JEG-3 human choriocarcinoma cell line with anti-human HLA-G (MEM-...
Product cited in: Boyson, Erskine, Whitman, Chiu, Lau, Koopman, Valter, Angelisova, Horejsi, Strominger: "Disulfide bond-mediated dimerization of HLA-G on the cell surface." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 25, pp. 16180-5, 2002 (PubMed).