HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) antibody

Details for Product No. ABIN94378
Antigen
Reactivity
Human
323
9
8
5
1
1
1
1
Host
Mouse
219
82
26
5
3
1
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
43
41
25
18
11
5
2
2
2
2
2
2
1
1
1
1
1
Application
Western Blotting (WB)
185
171
170
110
63
47
36
33
23
18
14
13
8
3
2
2
2
1
1
Options
Immunogen Recombinant human HLA-G denaturated heavy chain.
Clone MEM-G-4
Isotype IgG1
Specificity The antibody MEM-G/4 reacts with denaturated HLA-G heavy chain. The recognized epitope is present only on HLA-G1, -G2 and -G5 Molecules. HLA-G belongs to the MHC Class I molecules (MHC Class Ib, nonclassical) and it is expressed on the surface of trophoblast cells.
Cross-Reactivity (Details) Human
Purification Purified by protein-A affinity chromatography
Purity > 95 % (by SDS-PAGE)
Antigen
Alternative Name HLA-G (HLAG Antibody Abstract)
Pathways Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Human Leukocyte Antigen (HLA) in Adaptive Immune Response
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Supplier Images
Image no. 1 for anti-HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) antibody (ABIN94378) Western blotting analysis of HLA-G by the antibody MEM-G/4 on HLA-G1 transfectants (L...
Product cited in: Le Discorde, Le Danff, Moreau, Rouas-Freiss, Carosella: "HLA-G*0105N null allele encodes functional HLA-G isoforms." in: Biology of reproduction, Vol. 73, Issue 2, pp. 280-8, 2005 (PubMed).

Menier, Saez, Horejsi, Martinozzi, Krawice-Radanne, Bruel, Le Danff, Reboul, Hilgert, Rabreau, Larrad, Pla, Carosella, Rouas-Freiss: "Characterization of monoclonal antibodies recognizing HLA-G or HLA-E: new tools to analyze the expression of nonclassical HLA class I molecules." in: Human immunology, Vol. 64, Issue 3, pp. 315-26, 2003 (PubMed).