This Acetylcholinesterase antibody is un-conjugated
Application
Western Blotting (WB), ELISA, Immunoprecipitation (IP)
Cross-Reactivity (Details)
Cross reactivity against Acetyl Cholinesterase from other sources is unknown.
Purity
Anti-ACETYL CHOLINESTERASE is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Acetyl Cholinesterase [Bovine Erythrocytes].
Endotoxin Level
Low Endotoxin : No
Immunogen
Acetyl Cholinesterase [Bovine Erythrocytes] Immunogen Type: Native Protein
AChE
Reactivity: Rat
WB, IHC, ICC
Host: Rabbit
Polyclonal
unconjugated
Application Notes
ACETYL CHOLINESTERASE Antibody has been assayed against 1.0 µg of Acetyl Cholinesterase [Bovine Erythrocytes] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) code #605-4302 and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:1.200 to 1:5.000 of the reconstitution concentration is suggested for this product. ELISA Dilution: 1:5.000 - 1:20.000 IF Immunoprecipitation Dilution: 1:100 Western Blot Dilution: 1:500 - 1:5.000
Restrictions
For Research Use only
Format
Liquid
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Handling Advice
Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Storage
-20 °C
Storage Comment
Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.
Expiry Date
Expiration date is one (1) year from date of opening.