HLA-DR antibody
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- Target See all HLA-DR products
- HLA-DR
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Reactivity
- Human
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Host
- Rat
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Clonality
- Monoclonal
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Conjugate
- This HLA-DR antibody is un-conjugated
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Application
- Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Brand
- IHC-plus™
- Specificity
- Recognizes a monomorphic determinant of human HLA-DR antigen (MHC class II), expressed by B lymphocytes, activated T cells and by monocytes. Clone YE2/36 has also been reported to be cross reactive with mouse H-2 haplotypes b, d and q expressed by mouse strains including C57BL/6, DBA, and NZB. Reacts with paraffin embedded material following PLP (periodate-lysine-paraformaldehyde) fixation. This product is routinely tested in flow cytometry on human peripheral blood lymphocytes.
- Purification
- Affinity purified from tissue culture supernant
- Immunogen
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EHR-B Ramos cells.
Type of Immunogen: Cells - Clone
- YE2-36 HLK
- Isotype
- IgG2a
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- Application Notes
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Approved: Flo (1:50 - 1:100), IHC, IHC-Fr, IHC-P (10 μg/mL)
Usage: Flow Cytometry: This product is routinely tested in flow cytometry on human peripheral blood lymphocytes. Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunohistochemistry: This clone has been described reacting with paraffin embedded material following PLP (periodate-lysine-paraformaldehyde) fixation (2). - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- PBS with 0.09 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeat freeze-thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- Target
- HLA-DR
- Alternative Name
- HLA-DR (HLA-DR Products)
- Pathways
- Human Leukocyte Antigen (HLA) in Adaptive Immune Response
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