HLA-DR antibody

Catalog No. ABIN956622

The Rat Monoclonal anti-HLA-DR antibody (Clone YE2-36 HLK) (ABIN956622) specifically detects HLA-DR in FACS, IHC, IHC (fro) and IHC (p). The antibody is reactive with Human samples.

Quick Overview for HLA-DR antibody (ABIN956622)

Target: HLA-DR

Reactivity: Human

Host: Rat

Clonality: Monoclonal

Conjugate: This HLA-DR antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: YE2-36 HLK

Product Details anti-HLA-DR Antibody

Specificity: Recognizes a monomorphic determinant of human HLA-DR antigen (MHC class II), expressed by B lymphocytes, activated T cells and by monocytes. Clone YE2/36 has also been reported to be cross reactive with mouse H-2 haplotypes b, d and q expressed by mouse strains including C57BL/6, DBA, and NZB. Reacts with paraffin embedded material following PLP (periodate-lysine-paraformaldehyde) fixation. This product is routinely tested in flow cytometry on human peripheral blood lymphocytes.

Purification: Affinity purified from tissue culture supernant

Immunogen: EHR-B Ramos cells.

Type of Immunogen: Cells

Isotype: IgG2a

Application Details

Application Notes: Approved: Flo (1:50 - 1:100), IHC, IHC-Fr, IHC-P (10 μg/mL)

Usage: Flow Cytometry: This product is routinely tested in flow cytometry on human peripheral blood lymphocytes. Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunohistochemistry: This clone has been described reacting with paraffin embedded material following PLP (periodate-lysine-paraformaldehyde) fixation (2).

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS with 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for HLA-DR

Target: HLA-DR

Alternative Name: HLA-DR

Pathways: Human Leukocyte Antigen (HLA) in Adaptive Immune Response