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HLA-DR antibody

HLA-DR Reactivity: Human FACS, IHC, IHC (fro), IHC (p) Host: Rat Monoclonal YE2-36 HLK unconjugated
Catalog No. ABIN956622
  • Target See all HLA-DR products
    HLA-DR
    Reactivity
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    Human
    Host
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    Rat
    Clonality
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    Monoclonal
    Conjugate
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    This HLA-DR antibody is un-conjugated
    Application
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    Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Brand
    IHC-plus™
    Specificity
    Recognizes a monomorphic determinant of human HLA-DR antigen (MHC class II), expressed by B lymphocytes, activated T cells and by monocytes. Clone YE2/36 has also been reported to be cross reactive with mouse H-2 haplotypes b, d and q expressed by mouse strains including C57BL/6, DBA, and NZB. Reacts with paraffin embedded material following PLP (periodate-lysine-paraformaldehyde) fixation. This product is routinely tested in flow cytometry on human peripheral blood lymphocytes.
    Purification
    Affinity purified from tissue culture supernant
    Immunogen
    EHR-B Ramos cells.

    Type of Immunogen: Cells
    Clone
    YE2-36 HLK
    Isotype
    IgG2a
  • Application Notes
    Approved: Flo (1:50 - 1:100), IHC, IHC-Fr, IHC-P (10 μg/mL)

    Usage: Flow Cytometry: This product is routinely tested in flow cytometry on human peripheral blood lymphocytes. Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunohistochemistry: This clone has been described reacting with paraffin embedded material following PLP (periodate-lysine-paraformaldehyde) fixation (2).
    Comment

    Target Species of Antibody: Human

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    PBS with 0.09 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeat freeze-thaw cycles.
    Storage
    4 °C,-20 °C
    Storage Comment
    Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
  • Target
    HLA-DR
    Alternative Name
    HLA-DR (HLA-DR Products)
    Pathways
    Human Leukocyte Antigen (HLA) in Adaptive Immune Response
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