COL1A1 antibody

Catalog No. ABIN956759

Product Details anti-COL1A1 Antibody

Target: COL1A1 (Collagen, Type I, alpha 1 (COL1A1))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This COL1A1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Specificity: Specific for the propeptide portion of the ~180 kDa collagen I alpha 1 polypeptide in human lung fibroblast extract.

Purification: Affinity purified

Immunogen: peptide from the human collagen I alpha 1 propeptide sequence

Application Details

Application Notes: Approved: IHC (1:100), WB (1:1000)

Usage: The antibody also works well for immunohistochemistry on paraformaldehyde-fixed sections with a simple antigen-retrieval protocol (incubate slides for 20 minutes at 90° C in 10 mM sodium citrate (  pH 6.0)/ 0.1 % Tween-20). Note that in paraffin sections of formaldehyde-fixed fibrotic mouse lung tissue, the antibody recognizes collagen I molecules that are still associated with the cells in which they were synthesized.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

Handling Advice: Avoid repeated freeze thaw cycles.

Storage: -20 °C

Storage Comment: Store at -20°C, stable for 1 year. Avoid freeze-thaw cycles.

Target Details for COL1A1

Target: COL1A1 (Collagen, Type I, alpha 1 (COL1A1))

Alternative Name: COL1A1 / Collagen I Alpha 1 (COL1A1 Products)

Synonyms: OI4 antibody, Col1a-1 antibody, Cola-1 antibody, Cola1 antibody, Mov-13 antibody, Mov13 antibody, COLIA1 antibody, col1a1 antibody, MGC52532 antibody, COL1A1 antibody, COL1A2 antibody, alpha1(I) antibody, cb21 antibody, sb:cb21 antibody, sb:cb384 antibody, wu:fa99c12 antibody, wu:fb02c06 antibody, alpha3(I) antibody, col1a3 antibody, hm:zeh0348 antibody, wu:fa95h05 antibody, wu:fd02a10 antibody, wu:fj59a10 antibody, collagen type I alpha 1 chain antibody, collagen, type I, alpha 1 antibody, collagen, type I, alpha 1 S homeolog antibody, collagen alpha-1(I) chain antibody, collagen type I alpha 2 chain antibody, collagen, type I, alpha 1a antibody, collagen 1a1 antibody, collagen, type I, alpha 1b antibody, COL1A1 antibody, Col1a1 antibody, col1a1.S antibody, LOC397571 antibody, COL1A2 antibody, col1a1a antibody, col1a1 antibody, col1a1b antibody

Background: Name/Gene ID: COL1A1
Family: Collagen

Synonyms: COL1A1, Alpha-1 type I collagen, Collagen alpha 1 chain type I, Collagen alpha-1(I) chain, Collagen, type I, alpha 1, Collagen I, Pro-alpha-1 collagen type 1, OI4

Gene ID: 1277

UniProt: P02452

Pathways: Sensory Perception of Sound, Autophagy, Growth Factor Binding