ATG4D antibody (AA 444-457)

Catalog No. ABIN962638

Product Details anti-ATG4D Antibody

Target: ATG4D (Autophagy related 4D Cysteine Peptidase (ATG4D))

Binding Specificity: AA 444-457

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This ATG4D antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human ATG4D.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla (100%) Gibbon, Monkey, Marmoset, Panda, Dog, Horse (93%) Pig (86%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide CSQLAQPTLRLPRT from an internal region (near C-terminus) of human ATG4D (NP_116274.3). Percent identity by BLAST analysis: Human, Gorilla (100%), Gibbon, Monkey, Marmoset, Panda, Dog, Horse (93%), Pig (86%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 - 3.75 μg/mL), Peptide-ELISA (1:32000)

Usage: Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Preliminary experiments gave an approx. 26  kDa band in Human Skeletal Muscle lysates after 1 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 52.9  kDa according to NP_116274.3. The 26  kDa band was successfully blocked by incubation with the immunizing peptide.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for ATG4D

Target: ATG4D (Autophagy related 4D Cysteine Peptidase (ATG4D))

Alternative Name: ATG4D (ATG4D Products)

Synonyms: APG4-D antibody, APG4D antibody, AUTL4 antibody, 9830134P12Rik antibody, Apg4d antibody, Atg4dl antibody, Autl4 antibody, apg4d antibody, autophagy related 4D cysteine peptidase antibody, autophagy related 4D, cysteine peptidase antibody, autophagy related 4D, cysteine peptidase L homeolog antibody, ATG4D antibody, Atg4d antibody, atg4d.L antibody

Background: Name/Gene ID: ATG4D

Synonyms: ATG4D, APG4-D, APG4D, AUTL4, APG4 autophagy 4 homolog D, Cysteine protease ATG4D, Autophagin-4

Gene ID: 84971

NCBI Accession: NP_116274

UniProt: Q86TL0

Pathways: Autophagy