ERO1L antibody (AA 105-118)

Catalog No. ABIN962655

Product Details anti-ERO1L Antibody

Target: ERO1L (ERO1-Like Protein alpha (ERO1L))

Binding Specificity: AA 105-118

Reactivity: Human, Dog, Rabbit, Bat

Host: Goat

Clonality: Polyclonal

Conjugate: This ERO1L antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human ERO1L.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Elephant, Panda, Dog, Bat, Rabbit (100%) Marmoset, Mouse, Rat, Bovine, Horse, Pig (93%) Turkey, Platypus (86%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-QSDEVPDGIKSASY from an internal region of human ERO1L (NP_055399.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Elephant, Panda, Dog, Bat, Rabbit (100%), Marmoset, Mouse, Rat, Bovine, Horse, Pig (93%), Turkey, Platypus (86%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:32000), WB (0.1 - 0.3 μg/mL)

Usage: Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 70kD band observed in lysates of cell lines A431, HeLa, Jurkat (calculated MW of 54.4kD according to NP_055399.1). The observed molecular weight corresponds to the glycosylated form. Recommended concentration: 0.1-0.3 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for ERO1L

Target: ERO1L (ERO1-Like Protein alpha (ERO1L))

Alternative Name: ERO1A / ERO1L (ERO1L Products)

Synonyms: ERO1-L antibody, ero1-alpha antibody, ero1lb antibody, ERO1L antibody, ero1l antibody, ERO1-alpha antibody, ERO1A antibody, zgc:63959 antibody, endoplasmic reticulum oxidoreductase alpha L homeolog antibody, endoplasmic reticulum oxidoreductase 1 alpha antibody, endoplasmic reticulum oxidoreductase alpha antibody, ERO1-like (S. cerevisiae) antibody, ero1a.L antibody, ERO1A antibody, ero1a antibody, Ero1l antibody, Ero1a antibody

Background: Name/Gene ID: ERO1L

Synonyms: ERO1L, ERO1-like (S. cerevisiae), ERO1 (S. cerevisiae)-like, ERO1A, ERO1-alpha, ERO1-L-alpha, ERO1-like protein alpha, Oxidoreductin-1-L-alpha, ERO1-L

Gene ID: 30001

NCBI Accession: NP_055399

UniProt: Q96HE7

Pathways: Peptide Hormone Metabolism, ER-Nucleus Signaling, Brown Fat Cell Differentiation