PACSIN1 antibody (AA 2-15)

Catalog No. ABIN962676

Product Details anti-PACSIN1 Antibody

Target: PACSIN1 (Protein Kinase C and Casein Kinase Substrate in Neurons 1 (PACSIN1))

Binding Specificity: AA 2-15

Reactivity: Human, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This PACSIN1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human PACSIN1. Reported variants represent identical protein (NP_065855.1, NP_001186512.1).

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Pig (100%) Gibbon, Monkey, Marmoset, Goat, Panda, Dog, Bovine, Horse, Rabbit (93%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide SSSYDEASLAPEET-C from the N-terminus of human PACSIN1 (NP_065855.1). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Pig (100%), Gibbon, Monkey, Marmoset, Goat, Panda, Dog, Bovine, Horse, Rabbit (93%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 - 3.75 μg/mL), Peptide-ELISA (1:32000), WB (0.03 - 0.1 μg/mL)

Usage: Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 50 kDa band observed in Human Brain (hippocampus) lysates (calculated MW of 51.1 kDa according to NP_065855.1). Recommended concentration: 0.03-0.1 μg/mL. An additional band of unknown identity was also consistently observed at 37 kDa. This band was successfully blocked by incubation with the immunising peptide.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for PACSIN1

Target: PACSIN1 (Protein Kinase C and Casein Kinase Substrate in Neurons 1 (PACSIN1))

Alternative Name: Syndapin I / PACSIN1 (PACSIN1 Products)

Synonyms: MGC84055 antibody, sdpii antibody, pacsin1 antibody, x-PACSIN2 antibody, fe25d06 antibody, wu:fe25d06 antibody, zgc:114042 antibody, PACSIN1 antibody, SDPI antibody, A830061D09Rik antibody, H74 antibody, mKIAA1379 antibody, syndapin antibody, protein kinase C and casein kinase substrate in neurons 1 antibody, protein kinase C and casein kinase substrate in neurons 1 S homeolog antibody, protein kinase C and casein kinase substrate in neurons 2 antibody, protein kinase C and casein kinase substrate in neurons 1b antibody, PACSIN1 antibody, pacsin1.S antibody, pacsin2 antibody, pacsin1b antibody, Pacsin1 antibody

Background: Name/Gene ID: PACSIN1

Synonyms: PACSIN1, KIAA1379, Syndapin I, SDPI

Gene ID: 29993

NCBI Accession: NP_065855

UniProt: Q9BY11