Biotin antibody (HRP)

Catalog No. ABIN964581

Product Details anti-Biotin Antibody (HRP)

Target: Biotin

Reactivity: Please inquire

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Biotin antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Dot Blot (DB)

Specificity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Biotin coupled to sepharose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, biotinylated bovine serum albumin and Biotinylated IgG.

Sterility: Sterile filtered

Immunogen: Biotin conjugated to Keyhole Limpet Hemocyanin (KLH)
Immunogen Type: Other

Isotype: IgG

Application Details

Application Notes: Suitable for immunoblotting (western or dot blot), ELISA, immunoprecipitation and most immunological methods requiring high titer and specificity.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 100 µL

Concentration: 1.1 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: Gentamicin sulfate

Handling Advice: Do NOT add Sodium Azide!

Storage: 4 °C/-20 °C

Storage Comment: Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Expiry Date: 12 months

Target Details for Biotin

Target: Biotin

Abstract: Biotin Products

Target Type: Chemical

Background: Synonyms: HRP