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Biotin antibody (Alkaline Phosphatase (AP))

WB, ELISA, IHC, DB Host: Rabbit Polyclonal Alkaline Phosphatase (AP)

Catalog No. ABIN964582

Datasheet as PDF

Target See all Biotin products

Biotin

Reactivity

Please inquire
Host
54

34

26
Rabbit
Compatible Secondaries
Clonality
71

36
Polyclonal
Conjugate
29

9

7

7

7

4

3

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This Biotin antibody is conjugated to Alkaline Phosphatase (AP)
Application
54

46

43

33

30

19

14

13

12

12

9

8

6

4

4

3

2

2

2

2

2

1

1

1

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Dot Blot (DB)

Specificity

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Biotin coupled to sepharose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum and Biotinylated IgG.

Immunogen

Biotin conjugated to Keyhole Limpet Hemocyanin (KLH)
Immunogen Type: Other

Isotype

IgG
anti-Biotin antibody
WB, ELISA, IP, DB Host: Rabbit Polyclonal unconjugated

anti-Biotin antibody
ELISA Host: Goat Polyclonal unconjugated Fab fragment

anti-Biotin antibody
WB, FACS, IHC, IF, ISt, CM Host: Mouse Monoclonal Hyb-8 unconjugated

anti-Biotin antibody
IHC, ISt Host: Rabbit Monoclonal BTN-2032R unconjugated Recombinant Antibody

anti-Biotin antibody (DyLight 488)
WB, FM, FLISA Host: Goat Polyclonal DyLight 488

anti-Biotin antibody
FACS, ELISA Host: Mouse Monoclonal SB58c unconjugated

anti-Biotin antibody
WB, FACS, ELISA, IHC, IP, Lbl Host: Goat unconjugated

anti-Biotin antibody
IP, Con Host: Mouse Monoclonal unconjugated

anti-Biotin antibody
FACS, ELISA, IP, CyTOF Host: Mouse Monoclonal 1D4-C5 unconjugated

anti-Biotin antibody
IP, Con Host: Rabbit Polyclonal unconjugated

Application Notes

Suitable for immunoblotting (western or dot blot), ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.

Restrictions

For Research Use only
Format

Liquid

Concentration

0.5 mg/mL

Buffer

0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol, pH 8.0, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative

Sodium azide

Precaution of Use

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C

Storage Comment

Store vial at 4° C prior to opening. Restore with 1.0 ml of deionized water (or equivalent). DO NOT FREEZE. Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date for properly stored material is six (6) months from date of opening Expiration date is one (1) year from date of opening.

Expiry Date

12 months
Target

Biotin

Abstract

Biotin Products

Target Type

Chemical

Background

Synonyms: ALP, Alk Phos