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Biotin antibody (Alkaline Phosphatase (AP))

ELISA Host: Goat Polyclonal Alkaline Phosphatase (AP) F(ab')2 fragment

Catalog No. ABIN964587

Datasheet as PDF

Target See all Biotin products

Biotin

Fragment

F(ab')2 fragment

Reactivity

Please inquire
Host
53

34

27
Goat
Compatible Secondaries
Clonality
71

36
Polyclonal
Conjugate
29

9

7

7

7

4

3

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This Biotin antibody is conjugated to Alkaline Phosphatase (AP)
Application
55

46

43

33

31

19

14

13

12

12

9

9

6

4

4

3

2

2

2

2

2

1

1

1

1

1
ELISA

Specificity

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Biotin coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase, anti-Goat Serum and Biotinylated IgG. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c).

Immunogen

Biotin conjugated to Keyhole Limpet Hemocyanin (KLH)
Immunogen Type: Other

Isotype

IgG
anti-Biotin antibody
WB, ELISA, IP, DB Host: Rabbit Polyclonal unconjugated

anti-Biotin antibody
ELISA Host: Goat Polyclonal unconjugated Fab fragment

anti-Biotin antibody
WB, FACS, IHC, IF, ISt, CM Host: Mouse Monoclonal Hyb-8 unconjugated

anti-Biotin antibody
IHC, ISt Host: Rabbit Monoclonal BTN-2032R unconjugated Recombinant Antibody

anti-Biotin antibody (DyLight 488)
WB, FM, FLISA Host: Goat Polyclonal DyLight 488

anti-Biotin antibody
FACS, ELISA Host: Mouse Monoclonal SB58c unconjugated

anti-Biotin antibody
WB, FACS, ELISA, IHC, IP, Lbl Host: Goat unconjugated

anti-Biotin antibody
IP, Con Host: Mouse Monoclonal unconjugated

anti-Biotin antibody
FACS, ELISA, IP, CyTOF Host: Mouse Monoclonal 1D4-C5 unconjugated

anti-Biotin antibody
IP, Con Host: Rabbit Polyclonal unconjugated

Application Notes

This product has been assayed against 1.0 ug of Biotin conjuagted IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:500 to 1:2,000 of the reconstitution concentration is suggested for this product.

Restrictions

For Research Use only
Format

Liquid

Concentration

0.5 mg/mL

Buffer

0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol, pH 8.0, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative

Sodium azide

Precaution of Use

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C

Storage Comment

Store MIP-3 alpha Antibody Peroxidase Conjugated at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Expiry Date

12 months
Target

Biotin

Abstract

Biotin Products

Target Type

Chemical

Background

Synonyms: ALP, Alk Phos