STAT6 antibody (Internal Region, pTyr641)

Catalog No. ABIN964931

Product Details anti-STAT6 Antibody

Target: STAT6 (Signal Transducer and Activator of Transcription 6, Interleukin-4 Induced (STAT6))

Binding Specificity: Internal Region, pTyr641

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This STAT6 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Specificity: This affinity purified antibody is directed against human STAT6 pY641 protein. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography. A BLAST analysis was used to suggest cross-reactivity with STAT6 pY641 protein from human at 100% based on homology with the immunizing sequence. Reactivity against homologues from other sources is not known.

Characteristics: Anti-STAT6 phospho Y641 Antibody is ideal to study STAT6. STAT6 is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT6 is involved in interleukin-4 signaling. It interacts with NCOA1 via its C-terminal LXXLL motif. Further it induces the expression of BCL2L1/BCL-X(L), which is responsible for the anti-apoptotic activity of IL4. Knockout studies in mice suggested the roles of this gene in differentiation of T helper 2 (Th2) cells, expression of cell surface markers, and class switch of immunoglobulins. Following stimulation by IL-4 and IL-3, Stat6 is activated via phosphorylation at Tyr641 and translocates to the nucleus where it regulates cytokine-induced gene expression. STAT6 research areas include Immunology, Signal Transduction and Chromatin & Nuclear Signaling.

Purification: affinity purified

Sterility: Sterile filtered

Immunogen: STAT6 phospho Y641 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding Y641 of human STAT6 protein.
Immunogen Type: Peptide

Isotype: IgG

Application Details

Application Notes: This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 94.1kDa in size corresponding to STAT6 pY641 protein by western blotting in the appropriate cell lysate or extract.

Comment:

Gene Name: STAT6

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.13 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store vial at 4° C prior to opening. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Expiry Date: 12 months

Target Details for STAT6

Target: STAT6 (Signal Transducer and Activator of Transcription 6, Interleukin-4 Induced (STAT6))

Alternative Name: STAT6 (STAT6 Products)

Synonyms: D12S1644 antibody, IL-4-STAT antibody, STAT6B antibody, STAT6C antibody, signal transducer and activator of transcription 6 antibody, signal transducer and activator of transcription 6-like antibody, STAT6 antibody, Stat6 antibody, LOC100859543 antibody

Background: Anti-STAT6 phospho Y641 Antibody is ideal to study STAT6. STAT6 is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT6 is involved in interleukin-4 signaling. It interacts with NCOA1 via its C-terminal LXXLL motif. Further it induces the expression of BCL2L1/BCL-X(L), which is responsible for the anti-apoptotic activity of IL4. Knockout studies in mice suggested the roles of this gene in differentiation of T helper 2 (Th2) cells, expression of cell surface markers, and class switch of immunoglobulins. Following stimulation by IL-4 and IL-3, Stat6 is activated via phosphorylation at Tyr641 and translocates to the nucleus where it regulates cytokine-induced gene expression. STAT6 research areas include Immunology, Signal Transduction and Chromatin & Nuclear Signaling.
Synonyms: Signal transducer and activator of transcription 6 antibody, IL-4 Stat antibody

Gene ID: 6778

NCBI Accession: NP_001171549

UniProt: P42226

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response