Rabbit anti-Golden Syrian Hamster IgG (Heavy & Light Chain) Antibody (FITC) - Preadsorbed
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- Target See all IgG products
- IgG
- Fragment
- F(ab')2 fragment
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Binding Specificity
- Heavy & Light Chain
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Reactivity
- Golden Syrian Hamster
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- FITC
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Application
- Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)
- Specificity
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rabbit Serum, Golden Syrian Hamster IgG and Golden Syrian Hamster Serum.
- Characteristics
- This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
- Purification
- Preadsorption: Solid phase absorption
- Sterility
- Sterile filtered
- Immunogen
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Immunogen: Golden Syrian Hamster IgG whole molecule
- Isotype
- IgG
- Labeling Ratio
- 2.1
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- Application Notes
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Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
FLISA Dilution: 1:10,000 - 1:50,000
Flow Cytometry Dilution: 1:500 - 1:2,500
IF Microscopy Dilution: 1:1,000 - 1:5,000
- Comment
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Post Translational Modification: Phosphorylation
Excitation/Emission wavelength: 494 nm/514 nm - Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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Reconstitution Volume: 500 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- Concentration
- 1.0 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
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Product is photosensitive and should be protected from light.
Avoid cycles of freezing and thawing.
Dilute only prior to immediate use
This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20 °C or below after dilution. - Storage
- RT,4 °C,-20 °C
- Storage Comment
- Store vial at -20 °C or below prior to opening.
- Expiry Date
- 12 months
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- Target
- IgG
- Abstract
- IgG Products
- Target Type
- Antibody
- Background
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Synonyms: Rabbit F(ab')2 Anti-Golden Syrian & Armenian Hamster IgG Antibody Fluorescein conjugation, Rabbit F(ab')2 Anti-Hamster IgG Fluorescein Conjugated Antibody, Rabbit Fab2 Anti-Hamster IgG FITC Conjugated Antibody
Background: F(ab')2 Anti-Golden Syrian Hamster IgG Fluorescein Antibody was generated by enzymatic cleavage and subsequent separation from the Fc fragment. Because of their smaller size, F(ab)2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimental applications. F(ab)2 fragments penetrate tissue samples and show better antigen recognition and signal generation in IHC. F(ab)2 fragments lack the Fc region and therefore do not bind Fc receptors which effectively lowers background staining. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.
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