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Biotin antibody (Alkaline Phosphatase (AP))

WB, ELISA, IHC Host: Goat Alkaline Phosphatase (AP)

Catalog No. ABIN965464

Datasheet as PDF

Target See all Biotin products

Biotin

Reactivity

Please inquire
Host
53

34

27
Goat
Compatible Secondaries
Conjugate
29

9

7

7

7

4

3

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This Biotin antibody is conjugated to Alkaline Phosphatase (AP)
Application
54

46

43

33

30

19

14

13

12

12

9

9

6

4

4

3

2

2

2

2

2

1

1

1

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Specificity

This antibody reacts with Biotin

Cross-Reactivity (Details)

This antibody does not react with free BSA or free KLH

Characteristics

Concentration: 1.50 mg/ml (E 1% at 280 nm = 13.0)
Conjugate: Alkaline Phosphatase

Purification

Affinity purified using solid phase biotin

Purity

> 95 % based on SDS-PAGE

Immunogen

BSA-Biotin, KLH-Biotin
anti-Biotin antibody
WB, ELISA, IP, DB Host: Rabbit Polyclonal unconjugated

anti-Biotin antibody
ELISA Host: Goat Polyclonal unconjugated Fab fragment

anti-Biotin antibody
WB, FACS, IHC, IF, ISt, CM Host: Mouse Monoclonal Hyb-8 unconjugated

anti-Biotin antibody
IHC, ISt Host: Rabbit Monoclonal BTN-2032R unconjugated Recombinant Antibody

anti-Biotin antibody (DyLight 488)
WB, FM, FLISA Host: Goat Polyclonal DyLight 488

anti-Biotin antibody
FACS, ELISA Host: Mouse Monoclonal SB58c unconjugated

anti-Biotin antibody
WB, FACS, ELISA, IHC, IP, Lbl Host: Goat unconjugated

anti-Biotin antibody
IP, Con Host: Mouse Monoclonal unconjugated

anti-Biotin antibody
FACS, ELISA, IP, CyTOF Host: Mouse Monoclonal 1D4-C5 unconjugated

anti-Biotin antibody
IP, Con Host: Rabbit Polyclonal unconjugated

Application Notes

This Conjugate is suitable for all immunoassay applications. The optimal working dilution should be determined by the investigator. Suggested starting dilution(s): 1:500-1:2,000 for Immunohistochemistry and 1:50,000-1:5,000 for ELISA/Western blot

Comment

ALP: Enzyme derived from calf intestine (U.S. origin), Catalyzes the hydrolysis of phosphate groups from a substrate, which produces a colored reaction product or a release of light, The most common substrate for ALP is pNPP, which produces a soluble product, Unaffected by biological components in the ELISA assay, and produces consistent results, The reaction rate of ALP is linear, so its detection sensitivity can be increased by increasing the length of the reaction time. Detection OD of 405 nm

Restrictions

For Research Use only
Format

Liquid

Concentration

1.50 mg/mL

Buffer

30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free

Preservative

Sodium azide

Precaution of Use

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice

Prepare working dilution prior to use and then discard.

Storage

4 °C

Storage Comment

Store undiluted liquid at 2-8 °C. For storage at -20 °C, dilute with an equal volume of glycerol to prevent loss of enzymatic activity. Prepare working dilutions prior to use and then discard.
Target

Biotin

Abstract

Biotin Products

Target Type

Chemical