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Biotin antibody (HRP)

WB, ELISA, IHC Host: Goat HRP

Catalog No. ABIN965472

Datasheet as PDF

Target See all Biotin products

Biotin

Reactivity

Please inquire
Host
53

34

27
Goat
Compatible Secondaries
Conjugate
29

9

8

7

6

4

3

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This Biotin antibody is conjugated to HRP
Application
54

46

43

33

30

19

14

13

12

12

9

9

6

4

4

3

2

2

2

2

2

1

1

1

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Specificity

This antibody reacts with Biotin

Cross-Reactivity (Details)

This antibody does not react with free BSA or free KLH

Characteristics

Concentration: 1.0 mg/ml (E 1% at 280 nm = 13.0)
Conjugate: Horseradish Peroxidase

Purification

Affinity purified using solid phase biotin

Purity

> 95 % based on SDS-PAGE

Immunogen

BSA-Biotin, KLH-Biotin
anti-Biotin antibody
WB, ELISA, IP, DB Host: Rabbit Polyclonal unconjugated

anti-Biotin antibody
ELISA Host: Goat Polyclonal unconjugated Fab fragment

anti-Biotin antibody
WB, FACS, IHC, IF, ISt, CM Host: Mouse Monoclonal Hyb-8 unconjugated

anti-Biotin antibody
IHC, ISt Host: Rabbit Monoclonal BTN-2032R unconjugated Recombinant Antibody

anti-Biotin antibody (DyLight 488)
WB, FM, FLISA Host: Goat Polyclonal DyLight 488

anti-Biotin antibody
FACS, ELISA Host: Mouse Monoclonal SB58c unconjugated

anti-Biotin antibody
WB, FACS, ELISA, IHC, IP, Lbl Host: Goat unconjugated

anti-Biotin antibody
IP, Con Host: Mouse Monoclonal unconjugated

anti-Biotin antibody
FACS, ELISA, IP, CyTOF Host: Mouse Monoclonal 1D4-C5 unconjugated

anti-Biotin antibody
IP, Con Host: Rabbit Polyclonal unconjugated

Application Notes

This Conjugate is suitable for all immunoassay applications. The optimal working dilution should be determined by the investigator. Suggested starting dilution(s): 1:500-1:5,000 for Immunohistochemistry and 1:200-1:5000 for ELISA/Western blot

Comment

HRP: Enzyme derived from the plant horseradish, A small molecule that does not cause steric hindrance in antibody-antigen reactions, Highly sensitive, can detect low levels of antibody and can be used in a sensitive ECL assay, Hydrogen peroxide oxidizes chromogenic substrates such as TMB, ABTS, and OPD to produce a soluble product with a distinct color which can be measured by spectrophotometer, The reaction product has a high color stability after the reaction is stopped. Detection OD of 450 nm

Restrictions

For Research Use only
Format

Liquid

Reconstitution

Rehydrate with 1.1 mL of deionized water and let stand 30 minutes at room temperature to dissolve. Centrifuge to remove any particulates. Prepare fresh working dilution daily.

Concentration

1.0 mg/mL

Buffer

10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free

Preservative

Kathon CG

Storage

4 °C

Storage Comment

Store freeze-dried powder at 2-8 °C. Store lyophilized material at 2-8 °C. For long term storage after reconstitution, dilute with 50% glycerol and store at -20 °C as a liquid.
Target

Biotin

Abstract

Biotin Products

Target Type

Chemical