XRCC6 antibody (AA 506-541)

Catalog No. ABIN966469

This Mouse Monoclonal antibody specifically detects XRCC6 in WB, IF, IP and IHC (fp). It exhibits reactivity toward Human, Mouse, Monkey, Xenopus laevis and Hamster.It has been mentioned in 1 publication

Quick Overview for XRCC6 antibody (AA 506-541) (ABIN966469)

Target: XRCC6 (X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 6 (XRCC6))

Reactivity: Human, Mouse, Monkey, Xenopus laevis, Hamster

Host: Mouse

Clonality: Monoclonal

Conjugate: This XRCC6 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: N3H10

Product Details anti-XRCC6 Antibody

Binding Specificity: AA 506-541

Purification: Concentrated. Purified from the ascites fluid by Protein A chromatography

Immunogen: Human placental extract designated as PSE1-PL

Isotype: IgG2b

Application Details

Application Notes: Immunofluorescence
Immunoprecipitation (Native and denatured)(Use Protein A) (Ab 2µg/mg protein lysate)
Western blotting: (0.25-0.5 µg/ml for 2hrs at RT)
Immunohistology (Formalinparaffin)(Ab 1-2g/ml for 30 minute at RT)
Staining of formalin-fixed tissues is IMPROVED by boiling tissue sections in 10mM citrate buffer, pH 6.0.

Comment:

Cellular Localization: Nuclear.
Recommended Positive Control: BT474, HEP-G-2 or HeLa Cells, Tonsil

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL.

Buffer: 10mM PBS, pH 7.4, with 0.2% BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

References for anti-XRCC6 antibody (ABIN966469)

Wang, Chou, Blankson, Satoh, Knuth, Eisenberg, Pisetsky, Reeves: "Murine monoclonal antibodies specific for conserved and non-conserved antigenic determinants of the human and murine Ku autoantigens." in: Molecular biology reports, Vol. 18, Issue 1, pp. 15-28, (1993) (PubMed).

Target Details for XRCC6

Target: XRCC6 (X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 6 (XRCC6))

Alternative Name: Ku (p70)

Background: The Ku autoantigen is a heterodimer of 70kDa (p70) and ~80kDa (p80) proteins. The p70/p80 dimer is important for function of a 460kDa DNA-dependent protein kinase that phosphorylates certain transcription factors, including Sp1, Oct-1, p53, and SV40 large T antigen in vitro. Ku protein plays a role in cell signaling, proliferation, DNA repair, replication, transcriptional activation, and apoptosis.
Also available without BSA and azide at 1mg/ml.

Molecular Weight: 70kDa

Gene ID: 2547

Pathways: DNA Damage Repair