FYN antibody (FYN Oncogene Related To SRC, FGR, YES) (AA 1-132)

Details for Product anti-FYN Antibody No. ABIN967788, Supplier: Log in to see
Antigen
  • im:6908851
  • si:dkey-119m7.5
  • zgc:109996
  • c-fyn
  • cfyn-a
  • SLK
  • SYN
  • p59-FYN
  • PKC
  • fyn
  • zgc:86720
  • AI448320
  • AW552119
  • FYN proto-oncogene, Src family tyrosine kinase
  • FYN proto-oncogene, Src family tyrosine kinase S homeolog
  • FYN proto-oncogene, Src family tyrosine kinase b
  • FYN proto-oncogene, Src family tyrosine kinase L homeolog
  • FYN proto-oncogene, Src family tyrosine kinase a
  • Fyn proto-oncogene
  • FYN
  • fyn.S
  • fynb
  • fyn.L
  • Fyn
  • fyna
Alternatives
anti-Human FYN antibody for Immunocytochemistry
Epitope
AA 1-132
39
36
19
15
12
11
11
7
5
5
5
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Dog (Canine), Human, Rat (Rattus)
239
148
48
10
8
8
5
5
5
4
4
3
2
1
Host
Mouse
131
110
18
Clonality (Clone)
Monoclonal ()
Conjugate
This FYN antibody is un-conjugated
9
9
9
9
8
7
2
2
2
2
2
2
2
2
2
2
1
1
1
Application
Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
246
121
78
48
31
29
27
24
4
4
4
2
2
2
2
Options
Supplier
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Supplier Product No.
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Immunogen Human fyn aa. 1-132
Clone 25-FYN
Isotype IgG2b
Cross-Reactivity Dog (Canine), Rat (Rattus)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name Fyn (FYN Antibody Abstract)
Background Stimulation of the T cell antigen receptor (TcR) results in protein tyrosine phosphorylation via non-receptor tyrosine kinases. The src-related p59[fyn] kinase is one such enzyme that associates with the TcR. Ligation of the TcR activates the protein kinase activity of p59[fyn] in various human T cells. Fyn interacts with the CD3-zeta chains through its N-terminal region. In turn, fyn binds other proteins through its SH2 and SH3 domains. These proteins (p82 and p116) may serve as substrates and/or mediators of fyn activity. A 72 kDa fyn-related protein binds to the TcR/CD3 complex in certain T cells. This protein, known as p72[fyn-R], is a variant of the fyn kinase and contains at least four novel Ser/Thr phosphorylation sites. These sites may make p72[fyn-R] less susceptible to the action of phosphatases than p59[fyn]. Like p59[fyn], p72[fyn-R] is constitutively associated with its receptor. The exact relationship between these two proteins is not yet clear. However, p72[fyn-R] immunoprecipitates with various antisera raised to either the N- or C-terminus of fyn. Similar to src and yes, fyn is highly expressed in brain. The localization of fyn in mouse brain and its interaction with myelin-associated glycoprotein (MAG) suggests that the fyn kinase has a role in the sensory network and in myelination during early stages of CNS formation.
Molecular Weight 59 kDa
Pathways JAK-STAT Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Feeding Behaviour, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Activated T Cell Proliferation, Thromboxane A2 Receptor Signaling
Comment

Related Products: ABIN968537, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20° C.
Supplier Images
Western Blotting (WB) image for anti-FYN Oncogene Related To SRC, FGR, YES (FYN) (AA 1-132) antibody (ABIN967788) Western blot analysis of fyn on Jurkat cell lysate. Lane 1: 1:250, lane 2: 1: 500, la...
Immunofluorescence (IF) image for anti-FYN Oncogene Related To SRC, FGR, YES (FYN) (AA 1-132) antibody (ABIN967788) Immunofluorescent staining of A431 cells with anti-fyn antibody.
Product cited in: Zeng, Si, Yu, Le, Ng, Teng, Ryan, Wang, Ponniah, Pallen: "PTP alpha regulates integrin-stimulated FAK autophosphorylation and cytoskeletal rearrangement in cell spreading and migration." in: The Journal of cell biology, Vol. 160, Issue 1, pp. 137-46, 2003 (PubMed).

Feuillet, Semichon, Restouin, Harriague, Janzen, Magee, Collette, Bismuth: "The distinct capacity of Fyn and Lck to phosphorylate Sam68 in T cells is essentially governed by SH3/SH2-catalytic domain linker interactions." in: Oncogene, Vol. 21, Issue 47, pp. 7205-13, 2002 (PubMed).

Derkinderen, Toutant, Kadaré, Ledent, Parmentier, Girault: "Dual role of Fyn in the regulation of FAK+6,7 by cannabinoids in hippocampus." in: The Journal of biological chemistry, Vol. 276, Issue 41, pp. 38289-96, 2001 (PubMed).

Kawakami, Kawakami, Aaronson, Robbins: "Acquisition of transforming properties by FYN, a normal SRC-related human gene." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 85, Issue 11, pp. 3870-4, 1988 (PubMed).

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