XRCC4 antibody (AA 53-168)

Catalog No. ABIN968566

Product Details anti-XRCC4 Antibody

Target: XRCC4 (X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 4 (XRCC4))

Binding Specificity: AA 53-168

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This XRCC4 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human XRCC4 aa. 53-168

Clone: 4-XRCC4

Isotype: IgG2b

Application Details

Comment:

Related Products: ABIN968535, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References for anti-XRCC4 antibody (ABIN968566)

Gao, Sun, Frank, Dikkes, Fujiwara, Seidl, Sekiguchi, Rathbun, Swat, Wang, Bronson, Malynn, Bryans, Zhu, Chaudhuri, Davidson, Ferrini, Stamato, Orkin, Greenberg, Alt: "A critical role for DNA end-joining proteins in both lymphogenesis and neurogenesis." in: Cell, Vol. 95, Issue 7, pp. 891-902, (1999) (PubMed).

Modesti, Hesse, Gellert: "DNA binding of Xrcc4 protein is associated with V(D)J recombination but not with stimulation of DNA ligase IV activity." in: The EMBO journal, Vol. 18, Issue 7, pp. 2008-18, (1999) (PubMed).

Li, Otevrel, Gao, Cheng, Seed, Stamato, Taccioli, Alt: "The XRCC4 gene encodes a novel protein involved in DNA double-strand break repair and V(D)J recombination." in: Cell, Vol. 83, Issue 7, pp. 1079-89, (1996) (PubMed).

Target Details for XRCC4

Target: XRCC4 (X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 4 (XRCC4))

Alternative Name: XRCC4 (XRCC4 Products)

Synonyms: zgc:73312 antibody, MGC81443 antibody, homolog of human DNA ligase iv-binding protein XRCC4 antibody, 2310057B22Rik antibody, AW413319 antibody, AW545101 antibody, X-ray repair cross complementing 4 antibody, X-ray repair complementing defective repair in Chinese hamster cells 4 antibody, X-ray repair complementing defective repair in Chinese hamster cells 4 L homeolog antibody, DNA ligase IV-binding protein antibody, XRCC4 antibody, xrcc4 antibody, xrcc4.L antibody, Xrcc4 antibody

Background: DNA double-strand breaks (DSB) are generated during intrinsic eukaryotic DNA recombination events such as assembly of antigen receptor genes and meiotic and mitotic recombination. DSB repair proteins are also required to repair breaks induced by extrinsic factors such as ionizing radiation and mutagenic chemicals. DNA-PKcs, Ku70/Ku80, DNA ligase IV, and X-Ray Cross Complementation group 4 (XRCC4) are DSB proteins involved in both V(D)J recombination and DNA double-stranded break repair. XRCC4 activates DNA ligase IV and cells deficient in XRCC4 inefficiently form coding joints and signal joints during V(D)J recombination. XRCC4 contains a C-terminal nuclear localization sequence (NLS) and multiple phosphorylation sites, binds DNA, and is an effective substrate for DNA-PK. Phosphorylation of XRCC4 has no effect on its interactions with DNA ligase IV or end-joining activity, but can inhibit its DNA binding activity. Mice deficient in XRCC4 exhibit defects in lymphogenesis and apoptotic death of postmitotic neurons during neurogenesis. Thus, XRCC4 is a ubiquitous protein involved in DNA end joining during DNA recombination and repair, which is critical for cell growth and survival.
Synonyms: X-Ray Cross Complementation group 4

Molecular Weight: 55 kDa

Pathways: DNA Damage Repair, Production of Molecular Mediator of Immune Response