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GST antibody (Alkaline Phosphatase (AP))

GST Reactivity: Schistosoma japonicum WB, ELISA, IHC Host: Goat Polyclonal Alkaline Phosphatase (AP)
Rockland
Catalog No. ABIN99863
Supplier Product No.: 600-105-200
  • Target See all GST Antibodies
    GST (Glutathione S Transferase (GST))
    Reactivity
    • 95
    • 29
    • 14
    • 9
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    Schistosoma japonicum
    Host
    • 60
    • 54
    • 46
    • 2
    • 1
    • 1
    • 1
    Goat
    Clonality
    • 99
    • 64
    Polyclonal
    Conjugate
    • 74
    • 11
    • 11
    • 10
    • 5
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This GST antibody is conjugated to Alkaline Phosphatase (AP)
    Application
    • 147
    • 77
    • 37
    • 30
    • 26
    • 25
    • 15
    • 11
    • 9
    • 9
    • 8
    • 6
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
    Supplier Product No.
    600-105-200
    Supplier
    Rockland
    Purpose
    GST Antibody Alkaline Phosphatase Conjugated
    Cross-Reactivity (Details)
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, anti-Alkaline Phosphatase, as well as purified and partially purified Glutathione-S-Transferase [Schistosoma japonicum].
    Characteristics
    Synonyms: goat anti-GST antibody alkaline phosphatase conjugation, alk phos conjugated goat anti-GST antibody, Glutathione-S-Transferase, GST antibody, anti-GST antibody, anti-Glutathione-S-Transferase antibody, ALP, Alk Phos
    Purification
    Anti-GST Alkaline Phosphatase Conjugated antibody as prepared from monospecific antiserum by immunoaffinity chromatography using GST coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above.
    Sterility
    Sterile filtered
    Immunogen

    Immunogen: Glutathione-S-Transferase [Schistosoma japonicum]

    Immunogen Type: Native Protein

    Isotype
    IgG
    Top Product
    Discover our top product GST Primary Antibody
  • Application Notes

    Immunohistochemistry Dilution: 1:200 - 1:1,000

    Application Note: Anti-GST Antibody Alkaline Phosphatase conjugated has been tested by ELISA and western blot and is suitable for immunoblotting, ELISA, immunohistochemistry as well as other antibody based assays using alkaline phosphatase conjugates requiring lot-to-lot consistency. Optimal concentrations in these or other immunoassays should be determined by the researcher.

    Western Blot Dilution: 1:500 - 1:2,000

    ELISA Dilution: 1:1,000 - 1:5,000

    Other: User Optimized

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1.0 mg/mL
    Buffer

    Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.1 % (w/v) Sodium Azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
    Storage Comment
    Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
    Expiry Date
    12 months
  • Target
    GST (Glutathione S Transferase (GST))
    Alternative Name
    GST (GST Products)
    Background
    Background: GST Antibody is specific for the GST affinity tag. Affinity tags re appended to proteins thereby allowing them to be purified from their crude biological source using an affinity technique. Common affinity tags include glutathione-S-transferase (GST), chitin binding protein (CBP), maltose binding protein (MBP), and the poly-Histidine or HIS-tag.
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