GST antibody (Alkaline Phosphatase (AP))
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- Target See all GST Antibodies
- GST (Glutathione S Transferase (GST))
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Reactivity
- Schistosoma japonicum
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This GST antibody is conjugated to Alkaline Phosphatase (AP)
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
- Supplier Product No.
- 600-105-200
- Supplier
- Rockland
- Purpose
- GST Antibody Alkaline Phosphatase Conjugated
- Cross-Reactivity (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, anti-Alkaline Phosphatase, as well as purified and partially purified Glutathione-S-Transferase [Schistosoma japonicum].
- Characteristics
- Synonyms: goat anti-GST antibody alkaline phosphatase conjugation, alk phos conjugated goat anti-GST antibody, Glutathione-S-Transferase, GST antibody, anti-GST antibody, anti-Glutathione-S-Transferase antibody, ALP, Alk Phos
- Purification
- Anti-GST Alkaline Phosphatase Conjugated antibody as prepared from monospecific antiserum by immunoaffinity chromatography using GST coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above.
- Sterility
- Sterile filtered
- Immunogen
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Immunogen: Glutathione-S-Transferase [Schistosoma japonicum]
Immunogen Type: Native Protein
- Isotype
- IgG
- Top Product
- Discover our top product GST Primary Antibody
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- Application Notes
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Immunohistochemistry Dilution: 1:200 - 1:1,000
Application Note: Anti-GST Antibody Alkaline Phosphatase conjugated has been tested by ELISA and western blot and is suitable for immunoblotting, ELISA, immunohistochemistry as well as other antibody based assays using alkaline phosphatase conjugates requiring lot-to-lot consistency. Optimal concentrations in these or other immunoassays should be determined by the researcher.
Western Blot Dilution: 1:500 - 1:2,000
ELISA Dilution: 1:1,000 - 1:5,000
Other: User Optimized
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1.0 mg/mL
- Buffer
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Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.1 % (w/v) Sodium Azide - Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
- Storage Comment
- Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
- Expiry Date
- 12 months
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- Target
- GST (Glutathione S Transferase (GST))
- Alternative Name
- GST (GST Products)
- Background
- Background: GST Antibody is specific for the GST affinity tag. Affinity tags re appended to proteins thereby allowing them to be purified from their crude biological source using an affinity technique. Common affinity tags include glutathione-S-transferase (GST), chitin binding protein (CBP), maltose binding protein (MBP), and the poly-Histidine or HIS-tag.
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