|Antigen||BCL2-Antagonist/killer 1 (BAK1) Antibodies|
|Reactivity||Human, Mouse (Murine), Pig (Porcine), Rat (Rattus) Alternatives|
|Conjugate||This BAK1 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Western Blotting (WB)
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Product Details anti-BAK1 AntibodyTarget Details BAK1 Application Details Handling Images
|Specificity||This antibody detects Bcl-2-like 7 at N-term.|
|Purification||Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide|
|Immunogen||Peptide from N Terminus of the protein sequence according to NP_001179.1 Genename: BAK1|
Target Details BAK1Product Details anti-BAK1 Antibody Application Details Handling Images back to top
|Alternative Name||Bcl-2-Like 7 (BAK1 Antibody Abstract)|
|Background||Bak or Bcl2 homologous antagonist is a member of the Bcl2 family of proteins. The Bcl-2 related proteins interact with one another through the formation of homo and heterodimers. The susceptibility of cells to apoptotic stimuli is thought to be controlled by the relative ratios of the different Bcl2 family proteins. Bak has been demonstrated to accelerate the rate of apoptosis in growth factor deprived murine lymphoid, neuronal and fibroblastic cell lines. This protein localizes to mitochondria, and functions to induce apoptosis. It interacts with and accelerates the opening of the mitochondrial voltage-dependent anion channel, which leads to a loss in membrane potential and the release of cytochrome c. Bak also interacts with the tumor suppressor P53 after exposure to cell stress.Synonyms: Apoptosis regulator BAK, BAK, BAK1, BCL2L7, Bcl-2 homologous antagonist/killer, Bcl-2-like protein 7, Bcl2-L-7, CDN1, cell death inhibitor 1|
|Pathways||Apoptosis, Steroid Hormone Mediated Signaling Pathway, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Unfolded protein response|
Application DetailsProduct Details anti-BAK1 Antibody Target Details BAK1 Handling Images back to top
Peptide ELISA: Limit dilution 1: 32000. Western Blot: 0.5-1 μg/mL. Approx 25 kDa band seen in A431 cell lysate.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-BAK1 Antibody Target Details BAK1 Application Details Images back to top
|Buffer||Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.|