anti-Mouse (Murine) BID antibody for Western Blotting

Recommended BID Antibody (supplied by: Log in to see )

Antigen
BH3 Interacting Domain Death Agonist (BID) Antibodies
  • FP497
  • 2700049M22Rik
  • AI875481
  • AU022477
  • BID
  • bid
  • si:ch211-238n5.6
  • fp497
  • xbid
  • DKFZp469E066
  • BH3 interacting domain death agonist
  • BH3 interacting domain death agonist S homeolog
  • BID
  • Bid
  • bida
  • bid.S
  • bid
Reactivity
Mouse (Murine)
323
171
74
9
5
3
2
Host
Rabbit
295
97
20
9
Clonality
Polyclonal
Conjugate
This BID antibody is un-conjugated
21
19
14
9
8
7
4
4
3
3
3
3
3
3
3
3
3
2
1
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
354
190
126
90
50
41
36
25
14
11
11
6
3
2
1
1
1
1
1
1
1
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Catalog No. ABIN223028
$ 245.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.16301 ABIN3222829 IHC WB Rabbit pSer78 Log in to see Polyclonal 0
3.16301 ABIN3042321 ICC IHC (p) WB Rabbit IgG AA 1-195 Log in to see Polyclonal 0
3.16301 ABIN1532322 IHC ELISA WB Rabbit IgG AA 44-93 Log in to see Polyclonal 1
3.16301 ABIN388100 FACS IHC (p) WB Rabbit Ig Fraction AA 39-68, Ser61 Log in to see Polyclonal 3
3.16301 ABIN388099 IHC (p) WB Rabbit Ig Fraction AA 68-103, BH3 Domain Log in to see Polyclonal 3
3.16301 ABIN4284618 IHC IHC (p) IP WB Rabbit Log in to see Polyclonal 1
3.16301 ABIN4284615 IHC IHC (fro) IHC (p) IP WB Rabbit Log in to see Polyclonal 4
3.16301 ABIN1077862 ICC IHC WB Rabbit IgG AA 1-195 Log in to see Polyclonal 0
3.16301 ABIN968686 IF WB Mouse IgG1 AA 46-168 Log in to see 40-Bid 3
3.16301 ABIN3042950 IHC (p) WB Rabbit IgG AA 179-195, C-Term Log in to see Polyclonal 0
3.16301 ABIN1871288 IHC WB Rabbit IgG Log in to see Polyclonal 0
3.16301 ABIN151629 IHC IP WB Rabbit Log in to see Polyclonal 0
3.16301 ABIN153057 WB Rabbit pSer78 Log in to see Polyclonal 0
3.16301 ABIN153044 WB Rabbit AA 1-50 Log in to see Polyclonal 0
3.16301 ABIN408125 IHC (p) IHC WB Rabbit IgG Log in to see Polyclonal 0
3.16301 ABIN3183516 ELISA IHC WB Rabbit IgG Ser179 Log in to see Polyclonal 0
3.16301 ABIN3030163 ICC IHC (p) WB Rabbit IgG AA 1-195 Log in to see Polyclonal 0
3.16301 ABIN4284622 ICC IF WB Mouse IgG Log in to see 23F7 0
3.16301 ABIN1030888 ELISA WB Rabbit Middle Region Log in to see Polyclonal 0
3.16301 ABIN1030296 IHC ELISA WB Rabbit C-Term Log in to see Polyclonal 0

General

Antigen BH3 Interacting Domain Death Agonist (BID) Antibodies
Reactivity Mouse (Murine)
(323), (171), (74), (9), (5), (3), (2)
Host Rabbit
(295), (97), (20), (9)
Clonality
Polyclonal
Conjugate This BID antibody is un-conjugated
(21), (19), (14), (9), (8), (7), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Western Blotting (WB)
(354), (190), (126), (90), (50), (41), (36), (25), (14), (11), (11), (6), (3), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-BID Antibody

Target Details BID Application Details Handling References for anti-BID antibody (ABIN223028) Images
Blocking Peptide Blocking peptide for this product available: ABIN412617
Specificity The antibody detects the full length (22 kDa) mouse Bid.
Reactivity to other species has not been tested.
Purification Affinity purified
Immunogen Synthetic peptide surrounding amino acid 45 of mouse Bid
Isotype IgG

Target Details BID

Product Details anti-BID Antibody Application Details Handling References for anti-BID antibody (ABIN223028) Images back to top
Antigen
Alternative Name Bid (BID Antibody Abstract)
Background Bid, a BH3 domain-containing proapoptotic Bcl-2 family member, is localized in the cytosolic fraction of cells as an inactive precursor. Its active form is generated upon proteolytic cleavage by caspase-8 in the Fas signaling pathway. Cleaved Bid translocates to mitochondria and releases its potent proapoptotic activity, which in turn induces cytochrome c release and mitochondrial damage. The cytochrome c releasing activity of Bid was antagonized by Bcl-2. Mutation in the SH3 domain can diminish the cytochrome c releasing activity. In the animal model studies, Bid-deficient mice are found resistant to the lethal effects of death factor signals relayed throμgh Fas.
Synonyms: FP497 , MGC42355, MGC15319
Gene ID 12122
UniProt P70444
Pathways Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity

Application Details

Product Details anti-BID Antibody Target Details BID Handling References for anti-BID antibody (ABIN223028) Images back to top
Application Notes Western blot analysis (0.5-5 μg/mL) also works well in immunoprecipitation (20 μg/mL) and Immunohistochemistry (20 μg/mL). However, the optimal conditions should be determined individually. The antibody detects the full length (22 kDa) mouse Bid. Reactivity to other species has not been tested.
Restrictions For Research Use only

Handling

Product Details anti-BID Antibody Target Details BID Application Details References for anti-BID antibody (ABIN223028) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer 100 μg (0.5 mg/mL) affinity purified rabbit anti-Bid polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30 % glycerol, 0.5 % BSA, 0.01 % thimerosal.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice The antibody solution should be gently mixed before use.
Storage -20 °C
Storage Comment For long-term storage, aliquot and freeze at -70 °C.
Expiry Date 12 months

References for anti-BID antibody (ABIN223028)

Product Details anti-BID Antibody Target Details BID Application Details Handling Images back to top
Product cited in:

Huang, Masselli, Frisch, Hunton, Jiang, Wang: "Blockade of tumor necrosis factor-induced Bid cleavage by caspase-resistant Rb." in: The Journal of biological chemistry, Vol. 282, Issue 40, pp. 29401-13, 2007

Images

Product Details anti-BID Antibody Target Details BID Application Details Handling References for anti-BID antibody (ABIN223028) back to top
Supplier Images
Western Blotting (WB) image for anti-BH3 Interacting Domain Death Agonist (BID) antibody (ABIN223028) Western blot analysis of BID expression in 3T3 cell lysate.