We are preparing the requested document. Please wait, this may take a while...!
|Antigen||BH3 Interacting Domain Death Agonist (BID) Antibodies|
|Reactivity||Mouse (Murine) Alternatives|
|Conjugate||This BID antibody is un-conjugated Alternatives|
Western Blotting (WB)
|1 reference available|
|Supplier||Log in to see|
Product Details anti-BID AntibodyTarget Details BID Application Details Handling References for anti-BID antibody (ABIN223028) Images
|Blocking Peptide||Blocking peptide for this product available: ABIN412617|
The antibody detects the full length (22 kDa) mouse Bid.
Reactivity to other species has not been tested.
|Immunogen||Synthetic peptide surrounding amino acid 45 of mouse Bid|
Target Details BIDProduct Details anti-BID Antibody Application Details Handling References for anti-BID antibody (ABIN223028) Images back to top
|Alternative Name||Bid (BID Antibody Abstract)|
Bid, a BH3 domain-containing proapoptotic Bcl-2 family member, is localized in the cytosolic fraction of cells as an inactive precursor. Its active form is generated upon proteolytic cleavage by caspase-8 in the Fas signaling pathway. Cleaved Bid translocates to mitochondria and releases its potent proapoptotic activity, which in turn induces cytochrome c release and mitochondrial damage. The cytochrome c releasing activity of Bid was antagonized by Bcl-2. Mutation in the SH3 domain can diminish the cytochrome c releasing activity. In the animal model studies, Bid-deficient mice are found resistant to the lethal effects of death factor signals relayed throμgh Fas.
Synonyms: FP497 , MGC42355, MGC15319
|Pathways||Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity|
Application DetailsProduct Details anti-BID Antibody Target Details BID Handling References for anti-BID antibody (ABIN223028) Images back to top
|Application Notes||Western blot analysis (0.5-5 μg/mL) also works well in immunoprecipitation (20 μg/mL) and Immunohistochemistry (20 μg/mL). However, the optimal conditions should be determined individually. The antibody detects the full length (22 kDa) mouse Bid. Reactivity to other species has not been tested.|
|Restrictions||For Research Use only|
HandlingProduct Details anti-BID Antibody Target Details BID Application Details References for anti-BID antibody (ABIN223028) Images back to top
|Buffer||100 μg (0.5 mg/mL) affinity purified rabbit anti-Bid polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30 % glycerol, 0.5 % BSA, 0.01 % thimerosal.|
|Precaution of Use||This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||The antibody solution should be gently mixed before use.|
|Storage Comment||For long-term storage, aliquot and freeze at -70 °C.|
|Expiry Date||12 months|
References for anti-BID antibody (ABIN223028)Product Details anti-BID Antibody Target Details BID Application Details Handling Images back to top
|Product cited in:||
Huang, Masselli, Frisch, Hunton, Jiang, Wang: "Blockade of tumor necrosis factor-induced Bid cleavage by caspase-resistant Rb." in: The Journal of biological chemistry, Vol. 282, Issue 40, pp. 29401-13, 2007
ImagesProduct Details anti-BID Antibody Target Details BID Application Details Handling References for anti-BID antibody (ABIN223028) back to top