anti-Mouse (Murine) Caspase 8 antibody for Western Blotting

Recommended Caspase 8 Antibody (supplied by: Log in to see )

Antigen
Caspase 8, Apoptosis-Related Cysteine Peptidase (CASP8) Antibodies
  • casp8-A
  • xCaspase-8
  • casp8
  • CASP8
  • CASP-8
  • FLICE
  • MACH
  • Mch5
  • ALPS2B
  • CAP4
  • Casp-8
  • MCH5
  • caspase-8
  • zgc:92075
  • caspase 8 L homeolog
  • death related ced-3/Nedd2-like protein
  • caspase 8
  • caspase-8
  • caspase-8-like
  • caspase 8, apoptosis-related cysteine peptidase
  • casp8.L
  • LOC661095
  • CASP8
  • LOC5563818
  • casp8
  • LOC100222284
  • Casp8
Epitope
AA 430-482
40
14
14
12
11
11
11
9
8
7
7
7
6
5
5
5
5
4
4
4
4
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
345
134
104
21
21
3
1
Host
Rabbit
301
84
15
2
Clonality
Polyclonal
Conjugate
This Caspase 8 antibody is un-conjugated
20
14
7
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
369
124
111
53
51
34
31
26
20
12
12
8
7
5
2
1
1
1
1
1
Options
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Catalog No. ABIN724205
$ 329.45
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
33.641693 ABIN969003 FACS IHC ELISA WB Mouse IgG1 Log in to see 1H11 2
26.141695 ABIN2745525 FACS IHC ELISA WB Rat IgG1 Log in to see 1G12 0
26.141695 ABIN2745526 ICC FACS IHC ELISA WB Rat IgG1 Log in to see 3B10 0
20.141695 ABIN3183671 ELISA IHC WB Rabbit IgG Ser2 Log in to see Polyclonal 0
20.141695 ABIN3181527 IHC WB Mouse IgG1 Log in to see 2G12 0
20.141695 ABIN3188046 IHC WB Rabbit IgG Log in to see Polyclonal 0
20.141695 ABIN6137971 WB Rabbit Log in to see Polyclonal 0
19.8439 ABIN1846137 FACS IHC ELISA WB Mouse IgG1 Log in to see 0
19.8439 ABIN5647756 FACS IHC (p) WB Rabbit IgG AA 410-449 Log in to see Polyclonal 0
18.3439 ABIN3042601 WB Rabbit IgG AA 388-480 Log in to see Polyclonal 14
18.3439 ABIN6260484 ELISA ICC IF IHC WB Rabbit IgG Log in to see Polyclonal 4
16.8439 ABIN4880223 ELISA FACS IHC WB Mouse IgG1 Log in to see 1H11 0
16.8439 ABIN1105678 EIA FACS IHC (p) WB Mouse IgG1 Log in to see 1H11 0
16.684746 ABIN252118 ICC IHC (fro) IF IHC (p) IP IHC WB Rabbit Large Subunit, Small Subunit, active Log in to see Polyclonal 41
13.843901 ABIN6256281 ELISA ICC IF IHC WB Rabbit IgG pSer347 Log in to see Polyclonal 0
13.297794 ABIN4901141 ELISA FACS IHC (p) WB Mouse IgG1 Log in to see 1H11 0
13.297794 ABIN2135496 IHC ELISA WB Rabbit IgG pSer347 Log in to see Polyclonal 0
13.297794 ABIN5621419 WB Rabbit Log in to see Polyclonal 0
13.297794 ABIN5621378 WB Rabbit Log in to see Polyclonal 0
13.297794 ABIN2135492 WB Rabbit IgG Log in to see Polyclonal 0

General

Antigen Caspase 8, Apoptosis-Related Cysteine Peptidase (CASP8) Antibodies
Epitope AA 430-482
(40), (14), (14), (12), (11), (11), (11), (9), (8), (7), (7), (7), (6), (5), (5), (5), (5), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(345), (134), (104), (21), (21), (3), (1)
Host Rabbit
(301), (84), (15), (2)
Clonality
Polyclonal
Conjugate This Caspase 8 antibody is un-conjugated
(20), (14), (7), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(369), (124), (111), (53), (51), (34), (31), (26), (20), (12), (12), (8), (7), (5), (2), (1), (1), (1), (1), (1)
Pubmed 11 references available
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Product Details anti-Caspase 8 Antibody

Target Details Caspase 8 Application Details Handling References for anti-Caspase 8 antibody (ABIN724205) Images
Purification Purified by Protein A.
Immunogen KLH conjugated synthetic peptide derived from mouse Caspase-8 subunit p10
Isotype IgG
Plasmids, Primers & others

Target Details Caspase 8

Product Details anti-Caspase 8 Antibody Application Details Handling References for anti-Caspase 8 antibody (ABIN724205) Images back to top
Antigen
Alternative Name Caspase 8 (CASP8 Antibody Abstract)
Background

Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP1. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-|-AMC. Likely target for the cowpox virus CRMA death inhibitory protein.

Subcellular location: Cytoplasm

Synonyms: MACH, Mch5, FLICE, CASP-8, Caspase-8, Casp8

Gene ID 12370
UniProt O89110
Pathways Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Activation of Innate immune Response, Tube Formation, Positive Regulation of Endopeptidase Activity, Toll-Like Receptors Cascades

Application Details

Product Details anti-Caspase 8 Antibody Target Details Caspase 8 Handling References for anti-Caspase 8 antibody (ABIN724205) Images back to top
Application Notes WB 1:100-1000
IHC-P 1:100-500
IF(IHC-P) 1:50-200
IF(ICC) 1:50-200
Restrictions For Research Use only

Handling

Product Details anti-Caspase 8 Antibody Target Details Caspase 8 Application Details References for anti-Caspase 8 antibody (ABIN724205) Images back to top
Format Liquid
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20°C
Expiry Date 12 months

References for anti-Caspase 8 antibody (ABIN724205)

Product Details anti-Caspase 8 Antibody Target Details Caspase 8 Application Details Handling Images back to top
Product cited in:

Zhang, Zhao, Zhang, Hao, Yu, Min, Li, Ma, Chen, Yi, Tang, Meng, Liu, Wang, Shen, Zhang: "Decrease in male mouse fertility by hydrogen sulfide and/or ammonia can Be inheritable." in: Chemosphere, Vol. 194, pp. 147-157, 2018 Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Mouse (Murine)). Further details: Sample: testicular tissue

Tulsulkar, Glueck, Hinds, Shah: "Ginkgo biloba Extract Prevents Female Mice from Ischemic Brain Damage and the Mechanism Is Independent of the HO1/Wnt Pathway." in: Translational stroke research, Vol. 7, Issue 2, pp. 120-31, 2016

Akinrinde, Oyagbemi, Omobowale, Asenuga, Ajibade: "Alterations in blood pressure, antioxidant status and caspase 8 expression in cobalt chloride-induced cardio-renal dysfunction are reversed by Ocimum gratissimum and gallic acid in Wistar rats." in: Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements (GMS), Vol. 36, pp. 27-37, 2016 (Sample species: Rat (Rattus)). Further details: Sample: heart, kidney

Gao, Liu, Li, Wu, Peng, Jing: "Hispidulin induces mitochondrial apoptosis in acute myeloid leukemia cells by targeting extracellular matrix metalloproteinase inducer." in: American journal of translational research, Vol. 8, Issue 2, pp. 1115-32, 2016 Method employed by authors: Western Blotting (WB) (Sample species: Human).

Daverey, Agrawal: "Curcumin alleviates oxidative stress and mitochondrial dysfunction in astrocytes." in: Neuroscience, Vol. 333, pp. 92-103, 2016 Method employed by authors: Western Blotting (WB) (Sample species: Human).

Wang, Zhao, Yu, Feng, Zhang, Kou, Chu, Cui, Li, Zhang, Shen, Min: "Regulation of steroid hormones and energy status with cysteamine and its effect on spermatogenesis." in: Toxicology and applied pharmacology, Vol. 313, pp. 149-158, 2016 Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Sheep (Ovine)). Further details: Sample: testis

Zhao, Zhang, Liu, Zhang, Hao, Li, Chen, Shen, Tang, Min, Meng, Wang, Yi, Zhang: "Hydrogen Sulfide and/or Ammonia Reduces Spermatozoa Motility through AMPK/AKT Related Pathways." in: Scientific reports, Vol. 6, pp. 37884, 2016 Method employed by authors: Western Blotting (WB), Western Blotting (WB) (Sample species: Pig (Porcine)).

Fang, Zhang, Qi, Fan, Luo, Liu, Tan: "Evodiamine induces G2/M arrest and apoptosis via mitochondrial and endoplasmic reticulum pathways in H446 and H1688 human small-cell lung cancer cells." in: PLoS ONE, Vol. 9, Issue 12, pp. e115204, 2014 Method employed by authors: Western Blotting (WB) (Sample species: Human).

Wang, Han, Zhou, Wu, Wang, Jia, Lv, Cheng, Pan, Liu, Zhou, Sun: "Inhibition of hydrogen sulfide synthesis provides protection for severe acute pancreatitis rats via apoptosis pathway." in: Apoptosis : an international journal on programmed cell death, Vol. 18, Issue 1, pp. 28-42, 2013 Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Rat (Rattus)). Further details: Sample: pancreatic gland

Wu, Tang, Jiang, Li, Jiang, Liu: "PCSK9 siRNA inhibits HUVEC apoptosis induced by ox-LDL via Bcl/Bax-caspase9-caspase3 pathway." in: Molecular and cellular biochemistry, Vol. 359, Issue 1-2, pp. 347-58, 2011 Method employed by authors: Western Blotting (WB) (Sample species: Human).

Jeng, Dyson: "Direct measurement of the aspartic acid 26 pKa for reduced Escherichia coli thioredoxin by 13C NMR." in: Biochemistry, Vol. 35, Issue 1, pp. 1-6, 1996

Images

Product Details anti-Caspase 8 Antibody Target Details Caspase 8 Application Details Handling References for anti-Caspase 8 antibody (ABIN724205) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Caspase 8, Apoptosis-Related Cysteine Peptidase (CASP8) (AA 430-482) antibody (ABIN724205) Formalin-fixed and paraffin embedded human rectal carcinoma labeled with Anti-Caspase...
SDS-PAGE (SDS) image for anti-Caspase 8, Apoptosis-Related Cysteine Peptidase (CASP8) (AA 430-482) antibody (ABIN724205) L1 mouse liver lysates L2 mouse spleen lysates probed with Anti Caspase 8 Polyclonal ...
Western Blotting (WB) image for anti-Caspase 8, Apoptosis-Related Cysteine Peptidase (CASP8) (AA 430-482) antibody (ABIN724205) Image provided by the Independent Validation Program (badge number 29759). Lane 1: He...
Immunofluorescence (Cultured Cells) (IF (cc)) image for anti-Caspase 8, Apoptosis-Related Cysteine Peptidase (CASP8) (AA 430-482) antibody (ABIN724205) Image provided by One World Lab validation program. MCF-7 cells probed with Rabbit An...
Immunofluorescence (Cultured Cells) (IF (cc)) image for anti-Caspase 8, Apoptosis-Related Cysteine Peptidase (CASP8) (AA 430-482) antibody (ABIN724205) Image provided by One World Lab validation program. A431 cells probed with Rabbit Ant...
'Independent Validation' Badge
Lot Number 131127
Method validated Western Blotting
Positive Control HeLa cells
Negative Control c6/36 mosquito cells (non-reactive species)
Notes A strong band was observed at the correct molecular weight in the positive control sample. No major bands were observed in the negative sample.
Primary Antibody
  • Antigen: Caspase 8, Apoptosis-Related Cysteine Peptidase (CASP8) (1:200 dilution)
  • Catalog number: ABIN724205
  • Supplier: Log in to see
  • Supplier catalog number: Log in to see
  • Lot number: 131127
Secondary Antibody
  • Antigen: Goat Anti-Rabbit IgG (H + L)-HRP Conjugate (1:20,000 dilution)
  • Supplier: Log in to see
  • Catalog number: Log in to see
  • Lot number: L170-6515
Controls
  • Positive control: HeLa cell extract
  • Negative control: c6/36 cell extract
Protocol
  • 1. Total protein extracts were boiled in 1X SDS Sample Buffer containing 1% SDS and 1.25% β-mercaptoethanol at 95°C for 5 min prior to loading.
  • 2. 20 μg of boiled extracts were loaded and resolved on 8-16% SDS-polyacrylamide gel.
  • 3. The Thermo Scientific - Spectra Multicolor Broad Range (Cat # 26634) were used as molecular mass markers.
  • 4. Proteins were then transferred onto PVDF membrane by wet transfer and protein transfer was confirmed with Ponceau-S staining.
  • 5. The PVDF membrane was incubated with 25 mL of blocking buffer [Tris Buffered Saline, pH 7.4 plus 0.1% TW20 (TBST)] containing 5% (W/V) BSA at room temperature for 1 h.
  • 6. The membrane was rinsed with TBST once.
  • 7. The membrane was immersed with the protein side up in the primary antibody solution (CASP8; 1:200) in TBST containing 5% (W/V) BSA and incubated for 16 h at 4°C.
  • 8. The membrane was rinsed in TBST thrice for 5 min each.
  • 9. The membrane was incubated in the HRP-conjugated secondary antibody solution (Goat anti-rabbit IgG-HRP; 1:20,000) in TBST containing 5% (W/V) BSA and incubated for 1 hour at room temperature (~26°C) with gentle agitation.
  • 10. The membrane was rinsed thrice TBST thrice for 5 min each.
  • 11. The membrane was rinsed in TBS twice for 30 s each.
  • 12. Signals were detected with ECL-2 Substrate. The blot was scanned for 300 s.
  • 13. The membrane was rinsed three times TBST.
  • 14. Incubated in Acidic Glycine Stripping Buffer at room temperature with gentle agitation for 3 times, 10 min each.
  • 15. The membrane was washed in TBST 2 times for 10 min each.
  • 16. Repeated Steps 5-12 with the loading control antibody (anti-Actin; 1:6,000) and its matching secondary antibody (Goat anti-rabbit IgG-HRP; 1:20,000).
Experimental Notes - Nothing to note.
Validation Images
Western Blotting validation image for anti-Caspase 8, Apoptosis-Related Cysteine Peptidase (CASP8) (AA 430-482) antibody (ABIN724205) Figure 1: Western blot of lysates from HeLa cells (Lane 1), and c6/36 cells (Lane 2) ...