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|Antigen||DNA Fragmentation Factor, 40kDa, beta Polypeptide (Caspase-Activated DNase) (DFFB) Antibodies|
|Epitope||Intermediate Domain Alternatives|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This DFFB antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
|Supplier||Log in to see|
Product Details anti-DFFB AntibodyTarget Details DFFB Application Details Handling Images
|Specificity||DFF40 antibody was raised against a peptide corresponding to amino acids 203 to 218 of human DFF40.|
|Purification||Affinity chromatography purified via peptide column|
|Immunogen||Human DFF40 (Intermediate Domain) Peptide|
Target Details DFFBProduct Details anti-DFFB Antibody Application Details Handling Images back to top
|Alternative Name||DFFB / CAD (DFFB Antibody Abstract)|
|Background||Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A mouse DNase that causes DNA fragmentation was identified recently and designated CAD for caspase activated deoxyribonuclease (1,2). The human homologue of mouse CAD was more recently identified by three groups independently and termed CPAN, DFF40, and human CAD, respectively, (3-5). DFF45/ICAD is the inhibitory protein of DFF40/CAD (1,2,6) and forms complex with DFF40/CAD. Upon cleavage of DFF45/ICAD by activated caspase, DFF40/CAD is released and activated and eventually causes the degradation of DNA in the nuclei. Activation of DFF40/CAD, which causes DNA degradation, is the hallmark of apoptotic cell death.Synonyms: CPAN, Caspase-activated DNase, Caspase-activated deoxyribonuclease, Caspase-activated nuclease, DFF-40, DFF2, DFF40, DNA fragmentation factor 40 kDa subunit, DNA fragmentation factor subunit beta|
|Pathways||Apoptosis, Caspase Cascade in Apoptosis|
Application DetailsProduct Details anti-DFFB Antibody Target Details DFFB Handling Images back to top
ELISA. Western Blot: 1/500. A 40 kDa band can be detected. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-DFFB Antibody Target Details DFFB Application Details Images back to top
|Buffer||PBS containing 0.02 % sodium azide.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage Comment||Store the antibody undiluted at 2-8 °C.|