anti-Human KRT18 antibody for Simple Western

Recommended KRT18 Antibody

Antigen
Keratin 18 (KRT18) Antibodies
  • CK18
  • K18
  • Krt1-18
  • CYK18
  • DreK18
  • cb83
  • dapk1
  • sb:cb83
  • wu:fa13f02
  • wu:fb36b04
  • krt18
  • MGC64569
  • MGC75922
  • KRT18
  • keratin 18
  • keratin 18, type I L homeolog
  • keratin 18, type I
  • keratin, type I cytoskeletal 18
  • K18, simple type I keratin
  • Keratin, type I cytoskeletal 18
  • Krt18
  • KRT18
  • krt18
  • krt18.L
  • LOC700569
  • LOC100136778
  • k1c18
  • LOC100008885
Reactivity
Human
908
217
213
117
86
61
59
46
42
36
32
27
27
27
26
15
11
9
6
4
3
2
1
1
1
1
Host
Mouse
596
341
14
13
5
2
2
Clonality
Monoclonal
Conjugate
This KRT18 antibody is un-conjugated
56
42
18
11
11
11
11
11
11
10
9
9
8
8
8
8
8
8
8
8
8
8
8
8
8
8
3
3
3
3
3
2
1
Application
ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Simple Western (SimWes), Western Blotting (WB)
579
380
356
349
264
205
171
102
87
80
45
40
18
14
11
3
1
1
1
Options

Available images

Catalog No. ABIN4302634
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Clonality References Details
15.917077 ABIN4302637 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP SimWes WB Mouse IgG1 DC10 2
-13.157643 ABIN4302743 FACS IF IHC (p) SimWes Mouse IgG1 kappa DE-K18 0

General

Antigen Keratin 18 (KRT18) Antibodies
Reactivity Human
(908), (217), (213), (117), (86), (61), (59), (46), (42), (36), (32), (27), (27), (27), (26), (15), (11), (9), (6), (4), (3), (2), (1), (1), (1), (1)
Host Mouse
(596), (341), (14), (13), (5), (2), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This KRT18 antibody is un-conjugated
(56), (42), (18), (11), (11), (11), (11), (11), (11), (10), (9), (9), (8), (8), (8), (8), (8), (8), (8), (8), (8), (8), (8), (8), (8), (8), (3), (3), (3), (3), (3), (2), (1)
Application ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Simple Western (SimWes), Western Blotting (WB)
(579), (380), (356), (349), (264), (205), (171), (102), (87), (80), (45), (40), (18), (14), (11), (3), (1), (1), (1)
Supplier

Product Details anti-KRT18 Antibody

Target Details KRT18 Application Details Handling Images
Specificity This MAb reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It was reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18.
No Cross-Reactivity Mouse (Murine), Cow (Bovine), Sheep (Ovine), Dog (Canine), Hamster, Rat (Rattus), Pig (Porcine)
Purification Protein G purified
Immunogen Human breast cancer PMC 42 cells were used as immunogen to generate the Cytokeratin 18 (CK18) antibody.
Clone DA7
Isotype IgG1 kappa
Plasmids, Primers & others

Target Details KRT18

Product Details anti-KRT18 Antibody Application Details Handling Images back to top
Antigen
Alternative Name Cytokeratin 18 (KRT18 Antibody Abstract)
Background Gene Symbol: KRT18
Gene ID 3875
UniProt P05783
Pathways Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-KRT18 Antibody Target Details KRT18 Handling Images back to top
Application Notes Western Blot 1-3 μg/mL, Simple Western 10 μg/mL, Flow Cytometry 0.5-1 μg/million cells, ELISA 1-5 μg/mL for coating, Immunocytochemistry/Immunofluorescence 1-2 μg/mL, Immunoprecipitation 1-2 μg/500 μg protein lysate, Immunohistochemistry-Paraffin 0.5-1.0 μg/mLImmunoprecipitation and Immunofluorescence reported in literature. In Simple Western only 10 - 15 μL of the recommended dilution is used per data point. Separated by Size.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-KRT18 Antibody Target Details KRT18 Application Details Images back to top
Format Liquid
Buffer PBS with 0.05 % BSA.
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store at 4°C.

Images

Product Details anti-KRT18 Antibody Target Details KRT18 Application Details Handling back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Keratin 18 (KRT18) antibody (ABIN4302634) Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (DA7) [NBP2-29409] - Formalin-...
Simple Western (SimWes) image for anti-Keratin 18 (KRT18) antibody (ABIN4302634) Simple Western: Cytokeratin 18 Antibody (DA7) [NBP2-29409] - Electropherogram image o...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Keratin 18 (KRT18) antibody (ABIN4302634) Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (DA7) [NBP2-29409] - Formalin-...
Simple Western (SimWes) image for anti-Keratin 18 (KRT18) antibody (ABIN4302634) Simple Western: Cytokeratin 18 Antibody (DA7) [NBP2-29409] - Simple Western lane view...
Western Blotting (WB) image for anti-Keratin 18 (KRT18) antibody (ABIN4302634) Western Blot: Cytokeratin 18 Antibody (DA7) [NBP2-29409] - Analysis of Cytokeratin 18...
SDS-PAGE (SDS) image for anti-Keratin 18 (KRT18) antibody (ABIN4302634) SDS-Page: Cytokeratin 18 Antibody (DA7)