|Antigen||tyrosine 3-Monooxygenase/tryptophan 5-Monooxygenase Activation Protein, zeta Polypeptide (YWHAZ) Antibodies|
|Epitope||N-Term, acetylated Alternatives|
|Reactivity||Chicken, Cow (Bovine), Human, Mouse (Murine), Rat (Rattus), Sheep (Ovine) Alternatives|
|Conjugate||This YWHAZ antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|2 references available|
Product Details anti-YWHAZ AntibodyTarget Details YWHAZ Application Details Handling References for anti-YWHAZ antibody (ABIN318724) Images
|Specificity||This antibody recognizes the acetylated N-terminal of 14-3-3 zeta and the phosphorylated zeta isoform (also known as 14-3-3 delta) in all mammals. This antibody may not react with recombinant proteins that are not N-acetylated.|
Species reactivity (expected):Mammals.
Species reactivity (tested):Sheep, Bovine, Chicken, Rat, Human, Mouse.
|Immunogen||Synthetic peptide corresponding to acetylated N-terminal sequence of Sheep 14-3-3 zeta.|
|Plasmids, Primers & others|
Target Details YWHAZProduct Details anti-YWHAZ Antibody Application Details Handling References for anti-YWHAZ antibody (ABIN318724) Images back to top
|Alternative Name||14-3-3 Protein zeta/delta (YWHAZ Antibody Abstract)|
|Background||It is a member of the 14-3-3 family which consists of 30 kDa proteins that are involved in multiple protein kinase signaling pathways, regulation of cell cycle progression, cytoskeletal structure, transcription, intracellular trafficking and targeting. Protein interactions with 14-3-3 show distinct preference for its different isotypes and are regulated by phosphorylation of both 14-3-3 and the bound protein. 14-3-3 zeta is a susceptibility gene for paranoid schizophrenia and is overexpressed in the temporal cortex of cognitively impared Alzheimers patients. The protein binds yeast Sps1/Ste20-related kinase 1 (YSK1) and localizes to the golgi, possibly linking YSK1 signaling, protein transport, cell adhesion and migration. It co-localizes with epidermal growth factor receptor to the plasma membrane following epidermal growth factor signaling. The zeta isoform is involved in MAPKAPK2-mediated phosphorylation which may have a role in p38 MAPK-dependent inflammation. When bound to ADAM 22, 14-3-3 zeta is involved in cell adhesion. 14-3-3 zeta also interacts with cofilin, LIM-domain-containing protein kinase 1, protein kinase B/Akt and GPI alpha.Synonyms: KCIP-1, Protein kinase C inhibitor protein 1, YWHAZ|
|Pathways||Apoptosis, Hormone Transport, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Synaptic Membrane, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location|
Application DetailsProduct Details anti-YWHAZ Antibody Target Details YWHAZ Handling References for anti-YWHAZ antibody (ABIN318724) Images back to top
Immunohistochemistry on paraffin sections: requires antigen retrieval using heattreatment prior to staining of paraffin sections. ELISA. Western Blot: detects a band of approximately 30 kDa in HEK293 cell lysates.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-YWHAZ Antibody Target Details YWHAZ Application Details References for anti-YWHAZ antibody (ABIN318724) Images back to top
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
References for anti-YWHAZ antibody (ABIN318724)Product Details anti-YWHAZ Antibody Target Details YWHAZ Application Details Handling Images back to top
|Product cited in:||
Aitken: "14-3-3 proteins: a historic overview." in: Seminars in cancer biology, Vol. 16, Issue 3, pp. 162-72, 2006
Martin, Patel, Jones, Howell, Robinson, Aitken: "Antibodies against the major brain isoforms of 14-3-3 protein. An antibody specific for the N-acetylated amino-terminus of a protein." in: FEBS letters, Vol. 331, Issue 3, pp. 296-303, 1993
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